S-100蛋白新亚型在涎腺腺样囊性癌中的表达  

Expression of new S-100 protein subunits in adenoid cystic carcinoma

作　　者：黄健文[1] 

机构地区：[1]福建医科大学基础医学院病理系,福建省福州市350004

出　　处：《世界华人消化杂志》2001年第9期1027-1030,共4页World Chinese Journal of Digestology

摘　　要：目的观察 S-100 Al,2,4,6,B,K8.12,KLl,Vimentin,GFAP 和 NSE 在涎腺腺样囊性癌中的表达,探讨 S100蛋白新亚型的分布规律和作用机制以及肿瘤性肌上皮细胞的生物学特性.方法正常涎腺23例和腺样囊性癌26例常规40g·L^(-1)甲醛固定,石蜡包埋并制成4μm 厚的连续切片,行 HE 和免疫组化ABC 法染色.结果将腺样囊性癌中的瘤细胞分为 luminal tumor cells(LTCs)和 nonluminal tumor cells(NLTCs),也即肿瘤性肌上皮细胞.前者主要呈 S-100A、K8.12和 KLl 强阳性;后者主要表达 S-100B,GFAP,NSE 和 vimentin.结论 S-100蛋白新亚型在涎腺腺样囊性癌中的表达结果与多形性腺瘤相似.但是,前者中的肿瘤性肌上皮细胞无或很少表达 S-100新亚型,这是与良性肿瘤的鉴别点之一.此外,LTCs较 NLTCs 分化成熟些.AIM To observe the immunohistochemical expression of S- 100A1,S-100A2,S-100A4,S-100A6,S-100B,K8.12,KL1, Vimentin,GFAP and NSE in adenoid cystic carcinoma of salivary gland,and to evaluate the localization of S-100 proteins and biological behaviour of neoplastic myoepithelial cells. METHODS Twenty-three cases of normal salivary glands and 26 cases of adenoid cystic carcinoma were fixed in 10% formalin and embedded in paraffin.Serial section at 4 μm in thickness from the paraffin embedded blocks were used for the immunohistochemical studies following conventional histopathological studies. RESULTS Normal salivary glands had positive immunoreactivity for S-100A families,K8.12 and KL1 in the ductal cells,while S100B,GFAP and NSE were observed in peripheral nerve fibers innervating the gland.In adenoid cystic caicinoma,the tumor cells can be divided into luminal tumor cells(LTCs)and nonluminal tumor cells(NLTCs). Nonluminal tumor cells is neoplastic myoepithelial cells.The LTCs had positive immunoreactivity for S-100A subfamilies and cytokeratin.Nonluminal tumor cells had positive immunoreactivity for vimentin,GFAP and NSE. CONCLUSION The immunohistochemical expression of adenoid cystic carcinoma is similar to pleomorphic adenoma. However,neoplastic myoepithelial cells are negative for S- 100A families,which is the differentiation between benign and malignant tumors.

关 键 词：涎腺肿瘤 癌腺样囊性 神经组织蛋白质S100 免疫组织化学 ACC 

分 类 号：R739.8[医药卫生—肿瘤]