人类Vimentin基因第 1 ,2 ,6,7内含子序列的测定  

Introns 1,2,6,7 Sequencing of the Human Vimentin Gene

作　　者：李疆[1] 张清炯[1] 邝志和[1] 

机构地区：[1]中山医科大学中山眼科中心,广东广州510060

出　　处：《中山医科大学学报》2001年第5期335-337,共3页Academic Journal of Sun Yat-sen University of Medical Sciences

基　　金：广东省重点攻关项目 (99M 0 480 5G)基金资助课题;2 11重点学科基金资助 (980 0 1)

摘　　要：【目的】人vimentin(VIM )基因位于 10号染色体短臂 1区 3带 ,属于第Ⅲ型中间丝纤维蛋白基因。目前已知全部外显子序列及部分内含子序列 ,本文测定了部分未知的VIM基因内含子序列。【方法】通过已知VIM基因的外显子序列设计引物 ,用PCR扩增其未知的内含子片段 ,将PCR产物进行克隆测序。【结果】共测得未知的 4个内含子序列 ,内含子 1长6 92bp ,测得其全部序列 ;内含子 2长 2kb ,测得其两端与外显子相接的序列 ,共 1kb ;内含子 6长 45 6bp ,测得其全部序列 ;内含子 7长 40 4bp ,测得其全部序列。【结论】本研究PCR检测多例人VIM基因的内含子 7大小为 40 4bp ,与文献报道人类vi mentin基因的内含子 7大小为 80 0bp相差很大 ,但引物两端外显子序列相同。另外 ,文献报道的人类vimentin基因与外显子3相邻的第 2内含子末端 41nt的核苷酸序列与本研究所测的第Human vimentin(VIM) gene is located on the chromosome 10p13, belonging to the third type intermediate filament protein genes. Now the nucleotide sequence of all exons and part of introns of vimentin gene has already been known. Some unknown introns of vimentin gene were sequenced in this study. According to the sequence of VIM gene exon, primers were designed and the unknown introns were amplified by using PCR. Then the title segments were cloned and sequenced. Four unknown introns were sequenced: intron 1 was 692 bp, the sequence was all identified; intron 2 was 2 kb or so, the two side of the sequence was identified; intron 6 was 456 bp, the sequence was all identified; intron 7 was 404 bp, the sequence was all identified. [Conclusion] The length of VIM gene intron 7 is 404 bp, different from that of 800 bp reported in literature. The end 41 nt sequence of VIM gene intron 2 near exon 3 is also different from the sequence reported in literature.

关 键 词：VIM基因 遗传学 序列分析 DNA 内含子 测定 人类Vimentin基因 

分 类 号：Q987[生物学—遗传学] Q523[生物学—人类学]