外源性hRARβ基因对HL-60R细胞生长抑制和诱导分化作用  

The role of exogenous hRNAβ gene on the growth inhibition and differentiation induction of HL-60R cells

作　　者：陈睿博[1] 殷祥雷[2] 陈芳源[1] 韩洁英[1] 缪金明[1] 邵念贤[1] 欧阳仁荣[1] 顾建新[2] 

机构地区：[1]上海第二医科大学附属仁济医院血液科,上海血液学研究所白血病研究室,200001 [2]上海医科大学分子生物学研究室

出　　处：《中华血液学杂志》2001年第10期524-527,I010-1,共5页Chinese Journal of Hematology

摘　　要：目的　探讨hRARβ基因在白血病发病以及维甲酸类治疗中的细胞生物学作用。 方法　用电穿孔方法将 pcDNA3 hRARβ重组质粒导入缺失hRARβ基因表达的HL 6 0R细胞中 ,并用软琼脂集落形成、细胞形态学、硝基四氮唑蓝 (NBT)还原试验和流式细胞术等方法对其生物学特性进行研究。结果　建立了具有功能性hRARβ基因表达的HL6 0R hRARβ细胞株 ,在 0 .1μmol/LCh5 5作用下其生长抑制率为 5 0 % ,软琼脂培养集落形成率下降 ,细胞分化。结论　在人髓系白血病细胞中恢复外源性hRARβ基因的表达可以恢复该细胞对维甲酸类药物的敏感性 。Objective To investigate the role of hRNAβ gene in HL 60R cells and in their response to retinoids. Methods By electroporation, the recombinant pcDNA3 hRNAβ plasmid was transfected into the hRNAβ gene not expressed human myleocytic leukemic cell line HL 60R. In the presence of RAR selected agonist Ch55, the effect of RARβ gene on HL 60R was studied by the efficiency of colony formation in soft agar, cell morphology NBT reduction assay and flow cytometry. Results The HL60R hRARβ cells with stable hRNAβ gene expression were obtained. The growth and colony formation in soft agar of HL60R hRARβ cells were remarkably reduced as compared with that of their parent cells, and the cells were induced into granulocytic differentiation by Ch55 treatment at 10 -7 . Conclusion The expression of exogenous hRNAβ gene in HL 60R cells could resume their sensitivity to retinoids in inhibiting cell proliferation and inducing granulocytic differentiation.

关 键 词：白血病 hRARβ基因 维甲酸 HL-60R细胞系 生长抑制 诱导分化 治疗 

分 类 号：R73-34[医药卫生—肿瘤]