粘着斑激酶反义寡核苷酸对平滑肌细胞粘附迁移和凋亡的影响  

Effects of FAK antisense oligodeoxynucleotides on Smooth Muscle Cells Adhesion,Migration and Apoptosis

作　　者：尹航[1] 汪丽蕙[1] 彭旭[1] 霍勇[1] 夏春芳[1] 唐朝枢[1] 

机构地区：[1]北京大学第一医院心血管病研究所,北京100034

出　　处：《基础医学与临床》2001年第5期416-420,共5页Basic and Clinical Medicine

摘　　要：为研究粘着斑激酶反义寡核苷酸对平滑肌细胞粘附迁移和凋亡的调控作用 ,采用纤粘连蛋白 (fibronectin ,FN)诱导平滑肌细胞 (smoothmusclecells,SMCs)粘附迁移并活化粘着斑激酶 (focaladhesionkinase ,FAK) ,将FAK反义寡核苷酸 (antisenseoligodeoxynucleotides,ODNs)经脂质体转染细胞 ,观察对FAK磷酸化、细胞粘附迁移以及凋亡的影响。结果表明 ,FN在有效地诱导SMCs粘附迁移并活化FAK的同时 ,凋亡细胞数显著低于对照 ,(8 96± 1 2 ) %和(2 3 45± 9 6 ) %。脂质体可有效地介导ODNs转染 ,转染效率为 (86 7± 4 5 ) % ,FAK磷酸化表达量明显减少 ,不同浓度FN组 5～ 6 0mg L ,细胞铺展率减少 17 89%～ 2 7 6 7% ,10、2 0、40和 6 0mg LFN组迁移细胞数也分别显著减少2 3 2 6 %、2 1 6 3%、19 31%、17 88% ,凋亡细胞数比对照高 33 5 7% (P <0 0 5 ) ,也显著高于FN组。据此可以认为FAK活化及其介导的信号转导通路是细胞外基质诱导SMC粘附迁移并抑制其凋亡的重要因素。In order to study the effects of focal adhesion kinase(FAK) antisense oligodeoxynucleotides(ODNs) on smooth muscle cells(SMCs) adhesion, migration and apoptosis stimulated by fibronectin(FN), FAK antisense ODNs were transfected into SMCs by cationic lipid Results showed that FAK was expressed when SMCs adhesion and migration were successfully stimulated by FN, apoptosis was inhibited significantly by 40mg/L FN compared with control, (8 96±1 2)% vs (23 45±9 6)% FAK antisense ODNs were transfected efficiently by cationic lipid and FAK phosphorylation was inhibited significantly SMCs spreading rate in 5～60mg/L FN groups were reduced by 17 89～27 67% Cell migration stimulated by FN 10,20,40,60 mg/L was reduced by 23 26%,21 63%,19 31% and 17 88% respectively Apoptotic SMCs was 33 57% higher than the control( P <0 05) It is concluded that FAK phosphorylation and FAK mediated signal transduction play important roles in SMCs adhesion, migration and proliferation influenced by ECM The process can be effectively modulated by FAK antisense ODNs

关 键 词：粘着斑激酶 血管平滑肌细胞 迁移 凋亡 血管成形术 术后再狭窄 

分 类 号：R363[医药卫生—病理学]