全反式维甲酸对肺腺癌细胞株SPCA-1增殖及粘附分子表达的影响  

Effects of All－Trans Retinoic Acid on Proliferation of Lung Adenoma Cell Line SPCA－１and Expression of Adhesive Molecules

作　　者：於葛华[1] 许志祥[1] 徐颖[1] 张学光[1] 

机构地区：[1]苏州大学医学生物技术研究所免疫研究室,江苏苏州215007

出　　处：《癌症》2001年第10期1069-1071,共3页Chinese Journal of Cancer

基　　金：江苏省社会发展科技计划资助项目(BS99042)

摘　　要：目的:研究全反式维甲酸alltransretinoicacid,ATRA对肺腺癌细胞株SPCA1细胞的增殖及其表面粘附分子表达的影响。方法:使用1×10-6mol/L、1×10-5mol/L和2×10-5mol/L的ATRA及IL8、TNFα处理SPCA1细胞并培养72h后,用MTT方法观察细胞的增殖,间接免疫荧光法和流式细胞仪分析SPCA1细胞表面粘附分子的表达。结果:3种不同浓度ATRA都能抑制SPCA1细胞的增殖,抑制率分别为12.1%、30.5%、62.5%,且在浓度为1×10-6mol/L和1×10-5mol/L时与IL8和TNFα具有协同作用。3种不同浓度ATRA还可抑制SPCA1细胞表面CD11α、CD62E和CD62L粘附分子的表达;在浓度为1×10-6mol/L时抑制作用最明显,抑制率分别为84.6%、60.5%、62.0%;但对CD18和CD54分子的表达无明显作用。结论:ATRA能抑制SPCA1细胞增殖,降低其粘附分子的表达,提示ATRA对肺腺癌细胞的生长及转移可能有抑制作用。Objectives：To investigate the in hibitory effects of all－trans retinoic acid（ATRA）on the proliferation of lung adenoma cell line SPCA－１cells a nd the expression of adhesive molecu les on t he surface of the cells．Methods ：After incubation with IL－８and TNF－αfor ７ ２h，the effect of ATRA with concentrations of １×１０ －６ ，１×１０ －５ ，and ２×１０ －５ mol ／L respectively on the prolifera tion of SPCA－１cells was analyzed by MTT method．The expression of adhesiv e molecules on the surface of SPCA－１cell s was determined by flow cytometry．Results：The proliferation of SPCA－１cells was inhibited significantly a fter incubation with three differen t concentratio ns of ATRA．And the inh ibition rate was１２．１％，３０．５％，and ６２．５％re spectively．１×１０ －６ and １×１０ －５ mol ／L ATRA coordinating with IL－８an d TNF－αcan inhibit the proliferati on of SPCA－１cells．The expressions of CD１１α，CD６２E，and CD６２L can be si gnificantl y inhibited by three different concentrations o f ATRA．１×１０ －６ mol ／L ATRA was the most，and the inhib itory rate was ８４．６％，６０．５ ％，６２．０％respectively．but there were no effects on the expression of CD１ ８and CD５４．Conclusion：ATRA can inhibit the proliferation of SPC A－１cells a nd reduce the expressions of adhesive molecules on the surface of SPCA－１cells ．ATRA may be used in the control of the proliferation and metastasis of lung ad enoma cancer．

关 键 词：全反式维甲酸 肺腺癌细胞 细胞粘附分子 SPCA-1 细胞增殖 

分 类 号：R73-3[医药卫生—肿瘤]