人鼻咽癌细胞裸鼠体内演进中bcl-2、p53及c-erbB-2表达与EB病毒LMP1的关系  

Relationship between expression of bcl-2,p53,c-erbB-2 and EB virus LMP1 in human nasopharyngeal carcinoma cell progressing in nude mice

作　　者：张钦明[1] 陈燕[1] 陈小毅[1] 孙宁[1] 唐泽立[1] 李飞虹[1] 蔡琼珍[1] 

机构地区：[1]广东医学院病理学教研室,广东湛江524023

出　　处：《实用肿瘤杂志》2001年第6期374-378,共5页Journal of Practical Oncology

基　　金：广东省卫生厅科学研究基金课题(A199945 1);广东医学院青年科研基金资助 (青 970 2 )

摘　　要：目的　研究 EB病毒 L MP1在人高分化鼻咽癌细胞裸鼠体内演进中的作用及与 bcl- 2、p5 3及 c- erb B- 2蛋白表达的关系。方法　将表达 EB病毒 L MP1的人高分化鼻咽癌细胞及对照阴性细胞移植于裸鼠体内 ,然后将移植瘤细胞再移入裸鼠建立第二代移植瘤 ,如此传至第三代 ,分别观察移植瘤的成瘤和生长的变化 ,以免疫组化和图像分析技术检测移植瘤细胞 bcl- 2、p5 3及 c- erb B- 2蛋白表达。结果　表达 L MP1的鼻咽癌细胞原代移植成瘤率达 75 .0 %,比对照组显著增高 (P<0 .0 1)。平均潜伏期及体内倍增时间分别为 2 9.6± 7.7天和 2 .81± 0 .13天 ,比对照组明显缩短 (P<0 .0 1)。表达 L MP1的鼻咽癌细胞 (C1L)各代移植瘤的 bcl- 2蛋白表达阳性率显著低于对照组对应的各代细胞的表达 (P<0 .0 1) ;bcl- 2蛋白表达阳性率有显著下降趋势 ,对照组细胞 bcl- 2蛋白表达则呈峰值变化 ;各细胞系体内移植瘤细胞的 p5 3及 c- erb B- 2蛋白表达的变化趋势较为相似 ,即各细胞系不同代间 p5 3表达的阳性率有显著下降趋势 ,c- erb B- 2蛋白表达阳性率有显著上升趋势。表达 L MP1的鼻咽癌各代移植瘤细胞的 p5 3及 c- erb B- 2蛋白表达阳性率显著高于对照组对应的各代 (P<0 .0 1)。结论　 L MP1使鼻咽癌细胞的生长能力增强 。Objective To study the effect of EB virus LMP1 on progressing of human nasopharyngeal carcinoma cell(NPC) in nude mice and its relationship with the expression of bcl-2,p53,and c-erbB-2.Methods The LMP1 expression cells of human NPC and the negative control cells were implanted into nude mice for 3 generations.The change of tumorigenicity and growth of implant tumor were studied,the expression of bcl-2,p53,and c-erbB-2 of implant tumor were detected by immunohistochemical methods and image analysis.Results The auccessful implant was 75.0% in LMP1 expression cells,higher than that in control groups(P<0.01).The average latent period and double time of LMP1 expression group were 29.36± 7.7 days and 2.81±0.13 days respectively,shorter than those of control groups(P<0.01).With each passing generation in nude mice,there was a increasing tendency in the take rates,the average latent period and double time decreased.The expression of bcl-2 in all generations of LMP1 decreasing tendency of bcl-2 expression were observed with passing generations in nude mice,whereas the change of peak value was observed in control groups.The expression of p53 and c-erbB-2 had a similar tendency:the expression of p53 decreased and the expression of c-erbB-2 increased with generation passed in nude mice,but both were higher than those in their controls(P<0.01).Conclusion LMP1 can promote the growth and dedifferentration of NPC cells,which may be indused by a substituting pathway of bcl-2 expression,the accumulation and inactivation of p53 and the increasing expression of c-erbB-2,playing an important synergetic role in the progress of NPC.

关 键 词：鼻咽肿瘤 病理学 EB病毒 基因表达 基因调控 动物实验 BCL-2 P53 c-erbB-2 LMP1 

分 类 号：R739.63[医药卫生—肿瘤]