HPLC法测定麻黄汤分煎及合煎汤剂中甘草酸含量  

Determination of glycyrrhizic acid in MAHUANG DECOCTION by HPLC when decocted separately or as a whole

作　　者：曹佩雪[1] 梁光义[1] 徐必学[1] 靳凤云[2] 贺祝英[2] 

机构地区：[1]中国科学院天然产物化学重点实验室,贵州贵阳550002 [2]贵阳中医学院,贵州贵阳550002

出　　处：《中草药》2001年第11期981-983,共3页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目 ( 3996 0 0 83)

摘　　要：目的　测定麻黄汤分煎、合煎汤剂中甘草酸含量。方法　采用 HPL C法测定麻黄汤分煎与合煎液中甘草酸的含量。选 Hypersil C1 8柱 ,流动相 :乙腈 - 0 .1%乙酸水溶液 (33∶ 6 7) ,检测波长 2 5 4nm。结果　分煎液甘草酸平均回收率为 10 2 .43% ,RSD =2 .6 5 % ;合煎液甘草酸平均回收率为 99.41% ,RSD=3.11%。结论　方法简便、准确 。Object To determine the content of glycyrrihizic acid obtained when each individual ingredient in MAHUANG DECOCTION was decocted separately and then mixing the extracts with boiling water in comparison with that obtained by decocting the total composition together as a whole in the traditional way. Methods The contents of glycyrrhizic acid was determined by HPLC. Hypersil C 18 column was used, with acetonitrile∶0.1% acetic acid (33∶67) as the mobile phase and detected at the wavelength of 254 nm. Results The average recovery of glycyrrhizic acid when separately decocted was 102.43%, RSD=2.65%, while that of decoction in whole was 99.41%, RSD=3.11%. Conclusion The method was simple and accurate and was not interfered by other constituents in the prescription.

关 键 词：甘草酸 麻黄汤 HPLC 分煎汤剂 合煎汤剂 含量 中药 

分 类 号：R286.0[医药卫生—中药学]