血管内皮生长因子可溶性受体flt-1_(n3)对裸鼠人膀胱癌生长的影响  

The soluble receptor flt-1_(n3) of vascular endothelial growth factor inhibits bladder carcinoma angiogenesis and growth

作　　者：张志超[1] 张智清[2] 曾革非[2] 张立国[2] 郭应禄[1] 

机构地区：[1]北京大学第一医院,北京大学泌尿外科研究所,100034 [2]中国预防医学科学院病毒学研究所病毒基因工程国家重点实验室

出　　处：《中华泌尿外科杂志》2001年第8期460-463,共4页Chinese Journal of Urology

基　　金：国家 8 63高技术规划基金资助 (10 2 0 8 0 1 0 3 )

摘　　要：目的　研究血管内皮生长因子 (VEGF)受体flt 1胞外区基因抗肿瘤新生血管生成的作用。　方法　采用脂质体转染技术将含有flt 1胞外区前三个IgG样结构区域的真核表达质粒 pcD NA3.1/KDRn7转入人膀胱癌EJ细胞 ,用G418筛选得到稳定表达目的蛋白的细胞克隆 ,经固相结合实验筛选得到表达与VEGF特异结合的目的蛋白的细胞克隆 ,阳性细胞克隆进行PCR和RT PCR鉴定。对裸鼠人膀胱癌组织切片进行免疫组化染色 ,定量分析微血管密度。　结果　PCR和RT PCR结果显示重组质粒转入EJ细胞及在转录水平表达 ;免疫组化的微血管密度实验组明显低于阴性对照组。　结论　阻断VEGF/flt 1信号传导途径能够抑制肿瘤新生血管的形成 。Objective To verify whether the extracellular domain of flt 1 has anti angionesis activity in vivo. Methods A recombinant plasmid pcDNA 3.1/flt 1 n3 was transfected into the human bladder carcinoma EJ cell by lipofectamin, which was constructed by inserting the N terminal first three IgG like domain of VEGF receptor flt 1 (flt 1 n3 ) into eukaryotic expression vector pcDNA 3.1. Results The rflt 1 was functionally expressed in stably transfected EJ and the product secreted in the medium could be specifically bind to rhVEGF165.The result showed that the bladder cancer transfected with rflt 1 had a lower microvessle density than the control. Conclusions It is proved that the expressed rflt 1 n3 can inhibit tumor growth and angiogenesis in nude mice model.

关 键 词：膀胱肿瘤 癌 血管内皮生长因子受体 动物实验 

分 类 号：R737.14[医药卫生—肿瘤]