Clusterin在膀胱癌细胞中的表达及作用机制  

Clusterin expression in EJ cells and its antiapoptosis mechanism

作　　者：梁丽莉[1] 张强[1] 李钟[2] 曾荔[1] 丁义[1] 郭晓健[1] 张志文[1] 郭应禄[1] 

机构地区：[1]北京大学第一医院泌尿外科,北京大学泌尿外科研究所,100034 [2]美国芝加哥西北大学泌尿分子生物学系

出　　处：《中华泌尿外科杂志》2001年第8期499-501,共3页Chinese Journal of Urology

摘　　要：目的　探讨抗细胞凋亡因子Clusterin在膀胱癌EJ细胞中的表达及抗凋亡机制。　方法　应用免疫细胞化学染色检测Clusterin在EJ细胞中的表达 ,应用 3′末端脱氧核苷酰转移酶介导生物素标记法 (TUNEL)检测Clusterin对EJ细胞的抗凋亡作用。　结果　Clusterin在EJ细胞中有明显的阳性表达。细胞外加入Clusterin后 ,EJ细胞胞浆内染色加深 ,呈颗粒状 ,为强阳性染色。提示细胞外加入小剂量Clusterin可以明显抑制MMC诱导的EJ细胞凋亡作用。　结论　Clusterin在细胞外可以通过细胞膜进入细胞浆内发挥抗凋亡作用。检测Clusterin的表达特性有助于临床评估膀胱癌复发、耐药等生物学特性。Objective To study clusterin expression in bladder cancer cell line EJ and its antiapoptosis mechanism. Methods The expression of clusterin in EJ cells was detected by means of immunocytochemical staining and its effect on apoptosis studied by 3′TUNEL method. Results Clusterin expressed clearly in EJ cells.Likewise,when a small dose of clusterin was added,clusterin was stained strongly in the cytoplasm of the EJ cells and MMC induced apoptosis of the EJ cells was markedly inhibited. Conclusions Clusterin can penetrate the cell membrane getting into the cell cytoplasm and there in the cytoplasm the antiapoptosis effect is evoked.By detecting the clusterin expression,recurrence and multidrug resistance of a bladder cancer may be evaluated.It is hopeful that the use of clusterin antibody in the treatment of bladder cancer might be feasible.

关 键 词：膀胱肿瘤 癌 细胞凋亡 CLUSTERIN 抗细胞凋亡因子 

分 类 号：R737.14[医药卫生—肿瘤]