肾癌相关新基因GYLZ-RCC18反义寡核苷酸转染对肾癌细胞系GRC-1的作用  

The significance of transfection of GYLZ-RCC18 antisense oligonuclo tide on RCC cell line GRC-1

作　　者：张强[1] 艾军魁[1] 梁丽莉[1] 张志文[2] 郭晓健[1] 李钟[3] 那彦群[1] 郭应禄[1] 

机构地区：[1]北京大学第一医院,北京大学泌尿外科研究所,100034 [2]北京大学医学部生理系 [3]美国芝加哥西北大学泌尿分子生物学系

出　　处：《中华泌尿外科杂志》2001年第10期581-583,共3页Chinese Journal of Urology

基　　金：国家自然科学基金资助 (3 9870 841)

摘　　要：目的　探讨肾癌相关新基因GYLZ RCC18在肾癌发生发展中的作用机制。　方法　采用逆转录PCR方法检测GYLZ RCC18在肾癌和正常肾细胞系中的表达 ,将第一编码区起始处的2 0个碱基的反义和正义寡核苷酸导入肾癌细胞系GRC 1,连续观察对肾癌细胞生长、增殖凋亡、死亡率、形态学的影响。　结果　GYLZ RCC18在肾癌细胞系中表达明显高于正常肾细胞系 ;导入GYLZ RCC18反义寡核苷酸后 ,GRC 1细胞的核分裂可明显减少 ,导致GRC 1细胞死亡 ,抑制癌细胞的生长和增殖活性 ,并可特异诱导GRC 1连续凋亡。　结论　GYLZ RCC18是肾癌发生发展密切相关的癌基因 ,其表达可促进肾癌细胞的生长和增殖 ,并通过抗癌细胞死亡和凋亡机制对癌细胞起保护作用。Objective To investigate the effect and s ig nificance of GYLZ-RCC18 (Genebank accession number:BE825133) on RCC cell line GRC-1. Methods To detect the expression of GYLZ-RCC18 by means of RT-PCR in both the renal cell carcinoma cell line G RC-1 and the normal kidney cell line HK-2. 20bp GYLZ-RCC18 antisense oligon u clotide packed with liposome was transfected into GRC-1 cell.The change in gro wth speed,proliferation activity,apoptosis,mortality and morphology of GRC-1 w ere observed. Results The expression of GYLZ-RCC18 in R CC was much higher than in the normal kidney.After transfection of GYLZ-RCC18 a ntisense oligonuclotide,the mortality of GRC-1 increased significantly.At the s ame time the proliferation activity and growth speed were retarded remarkably.Th e antisense oligonuclotide induced apoptosis of GRC-1 throughout the observati on time. Conclusions GYLZ-RCC18,a RCC relative novel ge ne,overexpression would stimulate the growth and proliferation activity of RCC.A poptosis and mortality of the RCC cell were also retarded.So,transfection of ant isense oligonuclotide could inhibit the generation and development of RCC provid ing a new approach to the research of RCC.

关 键 词：肾肿瘤 癌 基因 GYLZ-RCC18反义苷酸转染 肾癌细胞系GRC-1 

分 类 号：R737.11[医药卫生—肿瘤]