TGFα和EGF对前列腺癌细胞系EGFR表达的调控作用  

Regulation of EGF-receptor expression by TGFα and EGF in human pros tate cancer cells

作　　者：严春寅[1] 陈卫国[1] 王晖[2] 浦金贤[1] 郭震华[1] 陈赐龄[1] 

机构地区：[1]苏州大学附属第一医院泌尿外科,215006 [2]日本国金泽大学肿瘤研究所

出　　处：《中华泌尿外科杂志》2001年第10期628-630,共3页Chinese Journal of Urology

基　　金：江苏省自然科学基金资助 (BK9915 1)

摘　　要：目的　探讨转化生长因子 (TGF)α和表皮生长因子 (EGF)对前列腺癌细胞系中表皮生长因子受体 (EGFR)表达的调控作用。　方法　采用RT PCR和Western印迹法分别对TGFα和EGF刺激前列腺癌细胞系LNCaP、PC3、ARCaP后EGFRmRNA表达及其蛋白水平进行定量分析。　结果　EGF引起前列腺癌细胞系LNCaP、PC3、ARCaP的EGFRmRNA升高 2～ 5倍 ,TGFα引起各细胞系EGFRmRNA升高 2～ 10倍。TGFα使各细胞系EGFR不同程度升高 ;EGF处理的LNCaPEGFR蛋白水平略升高 ,PC3、ARCaPEGFR蛋白水平降低。　结论　TGF/EGF EGFR通路在前列腺癌发生发展中起重要作用 ;非依赖型前列腺癌中TGFα EGFR自分泌环的作用可能强于EGFObjective To elucidate the regulation of e pidermal growth factor receptor (EGFR) expression by transforming growth factor (TGF)α and epidermal growth factor (EGF) in human prostate cancer cells. Methods EGFR mRNA expression and its protein level were measur ed by means of RT-PCR and Western blot respectively in three human prostate can cer cell lines,the androgen-responsive (LNCaP) and the nonresponsive (ARCaP and PC3), all being treated with exogenous EGF or TGFα. Results TGFα enhanced EGFR mRNA two-to tenfold in all three cell lines, whereas EGF increased EGFR mRNA two-to sixfold.In all the three cell lines, TGFα induc ed total EGFR protein levels were higher than EGF. Conclusions These data indicate that TGFα/EGF-EGFR path-way serves as a key growth regulator in prostate cancer.TGFα, but not EGF, would preferentially maintain an autocrine loop in human androgen-nonresponsive prostate cancer.

关 键 词：前列腺肿瘤 癌 多肽生长因子 受体 基因 转化生长因子 表皮生长因子 

分 类 号：R737.25[医药卫生—肿瘤]