透明颤菌血红蛋白基因在产PHB重组大肠杆菌中的引入  

INTRODUCTION OF VITREOSCILLA HEMOGLOBIN GENE IN A RECOMBINANT E.COLI FOR PHB PRODUCTION

作　　者：于慧敏[1] 史悦[1] 张延平[1] 杨胜利[2] 沈忠耀[1] 

机构地区：[1]清华大学化工系生物化工研究所,北京100084 [2]中国科学院上海生物工程研究中心,上海200233

出　　处：《微生物学报》2001年第5期548-552,共5页Acta Microbiologica Sinica

基　　金：国家"九五"攻关项目( 96 0 3 0 3 0 2 );国家自然科学基金重点项目 ( 2 9834 1 0 3);国家自然科学基金面上资助项目 ( 2 98760 2 1

摘　　要：将透明颤菌血红蛋白基因 (vgb)采用插入染色体的方式引入产聚 β 羟基丁酸酯(PHB)重组大肠杆菌VG1 (pTU1 4)中 ,以从分子水平上提高克隆菌对氧的利用率 ,解决PHB发酵生产过程中的供氧矛盾 ,透明颤菌血红蛋白的一氧化碳差光谱分析明表 ,vgb基因可以在VG1 (pTU1 4)中成功表达 ,且其表达量受溶氧水平的调控。vgb基因的引入可以同时促进菌体生长和PHB产品的积累 ,且溶氧水平越低 ,VHB表达量越高 。Vitreoscilla hemoglobin gene ( vgb ) was introduced into the recombinant Escherichia coli VG1(pTU14) for production of poly β hydroxybutyrate (PHB),in order to enhance the oxygen uptake of strain from molecular level and to solve the problem of oxygen limitation in process of fermentation. The carbon monoxide difference spectra analysis of Vitreoscilla hemoglobin (VHb) showed that vgb could be successfully expressed in VG1 (pTU14), and this expression was under the regulation of dissolved oxygen (DO).The higher amounts of VHb could be got correspond to the lower level of DO. Because of the introduction of vgb ,cell growth and PHB accumulation of VG1 (pTU14) were all strongly promoted during the process of batch culture.

关 键 词：透明颤菌 血红蛋白基因 聚Β-羟基丁酸酯 PHB 溶氧调控 大肠杆菌 好氧生物发酵 

分 类 号：Q753[生物学—分子生物学]