长蠹科几种检疫性害虫的ND4基因序列及系统进化  

Mitochondrial DNA ND4 sequence variation and phylogeny of five species of Bostrychidae (Coleoptera)

作　　者：黄永成 李伟丰 陆温[2] 陈永久[3] 张亚平[3] 

机构地区：[1]广西防城动植物检疫局,防城港538001 [2]广西大学农学院,南宁530005 [3]中国科学院昆明动物研究所细胞与分子进化开放研究实验室,昆明650223

出　　处：《昆虫学报》2001年第4期494-500,共7页Acta Entomologica Sinica

基　　金：广西壮族自治区科学研究与技术开发"九五"计划项目

摘　　要：长蠹科昆虫严重危害林木和仓贮物品。应用非损伤性DNA测序技术测定了来自不同国家的长蠹科害虫的线粒体DNAND4基因的部分序列。在获得的 2 0 4bp的序列中 ,5种昆虫的序列变异丰富 ,多数变异发生在密码子的第 3位点上。用PAUP3 1 1数据分析软件构建了 5个种的合意简约树。并将实验结果与形态学特征比较分析 ,探讨 5个种及所在属的系统进化。结果表明 :双钩异翅长蠹所在的异翅长蠹属分化最早 ,其次是竹大长蠹所在的大长蠹属、双棘长蠹和黑双棘长蠹所在的双棘长蠹属及红艳长蠹所在的钻木长蠹属。双棘长蠹和黑双棘长蠹隶属同一个属 ,遗传关系最近 ,分化最晚 ,与形态学研究结果相吻合。The Bostrychidae represent a highly destructive group of wood-boring and stored product beetles. We sequenced the mitochondrioal DNA gene encoding NADH dehydrogenase subunit 4(ND4) with non-invasive DNA Genotyping from 7 samples of 5 species of this family. The length of sequences was 204 bp. Sequence-comparison analysis indicated high variations in sequences of each species. The ND4 phylogenetic trees had been constructed for 5 Bostrychidae species with PAUP 3 1 1. This result was compared with the results based on the morphology of 5 species. Furthermore, the phylogenetic relationship among the species and genera that each species belongs to were discussed. The results indicated that H. aequalis of genus Heterobostrychus diverged the earliest, then did B. parallela of genus Bostrychopsis, S. anale and S.conigerum of genus Sinoxylon, X. religiosus of genus Xylothrips in turns. The two species of S. anale and S. conigerum were closely related, and they diverged last. This was basically identical with the results based on morphology.

关 键 词：长蠹科 ND4基因序列 MTDNA 系统进化 检疫性害虫 

分 类 号：S763.38[农业科学—森林保护学] Q969.496.2[农业科学—林学]