基于16s rRNA序列的新蚤属二齿新蚤种团部分种类的分子系统发育关系  

The molecular phylogeny of some species of the bidentatiformis group of the genus Neopsylla based on 16s rRNA gene

作　　者：鲁亮[1] 吴厚永[1] 

机构地区：[1]军事医学科学院微生物流行病研究所,北京100071

出　　处：《昆虫学报》2001年第4期548-554,共7页Acta Entomologica Sinica

基　　金：国家自然科学基金资助项目 (30 0 70 6 79) ;军事医学科学院院创新基金项目

摘　　要：通过比较线粒体基因组 16srRNA基因片段的差异 ,研究了分布于我国的二齿新蚤种团 (bi dentatiformisgroup) 7种新蚤的分子系统发育关系 ,以斯氏新蚤种团的特新蚤作为外群。结果显示 ,其中 5个种 (Neopsyllabidentatiformis、N .mana、N .pleski、N .teratura和N .hongyangensis)的种间序列差异小于 1%,变异水平和种内变异相当 ,显示了它们之间较近的亲缘关系 ;N .abagaitui和上述 5种之间有约 4 %的变异 ,说明该种有较长的分化历史。来自两个地区的二齿新蚤 4个样本间有两种不同的基因型 ,而红羊新蚤可以归入其中的一种。加上形态特征的特点 ,可以认为红羊新蚤不应该属于毛新蚤种团 ,而属于二齿新蚤种团 ;至于其是否为二齿新蚤的同种异名 ,还有必要做进一步的研究。另外 ,N .siboi和形态近缘种N .teratura之间存在 2 0 %的变异 ,其原因有待进一步研究。The molecular phylogeny of seven species of the bidentatiformis group in the genus Neopsylla was studied based on the mutation of 16s rRNA gene in mitochondrial genome. The results showed that the interspecies mutation rate of five species (N.bidentatiformis, N.mana, N.pleski, N.teratura and N.hongyangensis) was 1%, the same as the intraspecies mutation, suggesting that the relations among these species are closer; while the mutation rate of N.abagatui is 4%, suggesting the long diversity history of N.abagatui.Four samples of N.bidentatiformis from two localities showed two genotypes, one of which was also found in N.hongyangensis.As suggested also by the morphological character, N.hongyangensis should not be classified to the setosa group. Whether N.hongyangensis and N.bidentatiformis are the same species needs further study. N.siboi showed unexpected large mutation rate (20%) compared with morphologically related species N.teratura, and the reason for this is not clear.

关 键 词：新蚤属 二齿新蚤种团 16s RRNA 分子系统发育关系 

分 类 号：R384.3[医药卫生—医学寄生虫学] Q969.47[医药卫生—基础医学]