致病性迟缓爱德华菌的检测  

DETECTION OF PATHOGENIC EDWARSIELLA TARDA

作　　者：熊清明[1] 陆承平[1] 

机构地区：[1]南京农业大学农业部动物疫病诊断与免疫重点开放实验室,南京210095

出　　处：《微生物学报》2001年第6期736-740,共5页Acta Microbiologica Sinica

基　　金：江苏省"九五"重点攻关项目 (BE964 2 7)

摘　　要：系统检测了 2 5株迟缓爱德华菌 (Edwardasiellatarda ,Et)的胞外产物 (ECP) ,包括溶血素、胞外蛋白酶 (ECPase) ,并用Et强致病株JEL4的ECP抗血清作Dot ELISA检测各株Et的ECP ,同时对小鼠和剑尾鱼作致病性试验。试验表明 ,具动物致病性的菌株溶血素检测均为阳性 ,ECPase与致病性无关。JEL4ECP的Dot ELISA结果与动物致病性的符合率达 1 0 0 %。在此基础上 ,建立了致病性Et的检测方法 ,该方法只需作简便的平板溶血试验和Dot ELISA ,即可检测Et的ECP ,无须作动物试验 ,从而简化了致病性Et的检测程序 ,为研制检测试剂盒奠定了基础。Edwardsiella tarda(Et) strains had been detected both on their viruslent factor Excellular Product (ECP), including the hemolysin and extracellular protease (ECPase), and on their pathogenicity to mice and Xiphophorus helleri.ECP was detected by Dot-ELISA with rabbit antiserum against ECP of reference strain JEL4. The results showed that the animal pathogenicity of Et had good correlation with its hemolysin other than with ECPase. The agreement between Dot-ELISA of JEL4 ECP and pathogenicity to animal was up to 100%.It was desirable to establish a detecting method,which only need detect the ECP with plate assay (PA) and Dot-ELISA,but needn′t have animal experiment.Furthermore it is possible to develop a diagnosis kit of application to simplify the detecting procedure of pathogenic Et.

关 键 词：迟缓爱德华菌 溶血素 胞外蛋白酶 DOT-ELISA 检测 水产动物 

分 类 号：S852.61[农业科学—基础兽医学]