猪苓多糖对膀胱癌细胞钙离子浓度的影响  

Dynamic observation of the effects of polyporus polysaccharide on intracellular calcium in cultured human T24 cells with laser scanning confocal microscopy

作　　者：章国来[1] 曾星[1] 梅玉屏[1] 何盛琪[1] 张新梅[1] 黄羽[1] 

机构地区：[1]广州中医药大学附属广东省中医院,广州510120

出　　处：《中国临床药理学与治疗学》2001年第3期204-206,共3页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：国家自然科学基金资助项目(№ 3 9870 944 );教育部优秀青年教师资助项目 (教人司 [2 0 0 1] 3 9号 )

摘　　要：目的　观察猪苓多糖对人T2 4膀胱癌细胞内钙离子 (Ca2 +)浓度的影响 ,探讨猪苓多糖抗癌机理。方法　体外培养人T2 4细胞经钙荧光指示剂 (calciumcrimson AM )负载后 ,用激光扫描共聚焦显微镜测定猪苓多糖作用前后细胞内Ca2 +随时间的变化。结果　猪苓多糖低剂量 (0 .2mg·L- 1)使T2 4细胞内Ca2 +降低 ,高剂量 (>1mg·L- 1)使T2 4细胞内Ca2 +升高 ,最后维持在高钙水平上。猪苓多糖主要促进细胞核内Ca2 +升高 ,细胞浆Ca2 +无明显改变。结论　猪苓多糖可引起T2 4细胞内Ca2 +水平的显著变化 ,而且这种变化主要表现在细胞核内 。Aim To investigate the relationship between polyporus polysaccharide (PUPS) and changes of intracellular calcium in human T24 bladder cancer cells.Methods Human T24 cells were cultured with PUPS and intracellular calcium level was measured with the fluorescent Ca 2+ indicator calcium crimson AM and the laser scanning confocal microscopy.Results After human T24 cells were treated with a low dose of PUPS ( 0.2 mg·L -1 ),the intracellular calcium was decreased; while with PUPS (> 1.0 mg·L -1 ),the intracellular calcium became increased,and kept on a higher level.Conclusion PUPS participates in the process of calcium message transduction.

关 键 词：猪苓多糖 膀胱癌细胞 钙离子 细胞凋亡 抗肿瘤 

分 类 号：R737.14[医药卫生—肿瘤] Q279[医药卫生—临床医学]