血栓调节蛋白核心片段的基因克隆、表达及鉴定  

Gene Cloning, Expression and Identification of Core Fragment of Thrombo-modulin

作　　者：张淑敏[1] 王学谦[1] 李增刚[2] 董峥[3] 闫丽娜[3] 安士萍[3] 

机构地区：[1]天津市人民医院,天津300211 [2]天津市第一中心医院,天津300192 [3]天津市血液中心,天津300110

出　　处：《中国生物制品学杂志》2001年第4期200-203,共4页Chinese Journal of Biologicals

摘　　要：目的 获得血栓调节蛋白及其核心片段，以便研制临床诊断治疗制品。方法 应用ＰＣＲ获得血栓 调节蛋白ＥＧＦ４～６的基因片段，构建克隆载体ＥＧＦ４－６／ＰＹ和表达载体ＥＧＦ４－６／ＰＢＶ２２０），表达血栓调节蛋白ＥＧＦ４-６。结果 原核细胞表达量约占菌体可溶性蛋白的１２％，经ＫＰＴＴ试剂盘检测具有生物学活性。结论 为进一步 研究临床诊断治疗制剂创造了条件。Objective To obtain thrombomodulin(TM) and the core fragment of it for clinical diag-nosis and treatment. Methods The EGF4-6 gene fragment of TM was amplified by PCR and used for the construction of clone vector EGF4-6/pT and expression vector EGF4-6/pBV220. The biological activity of TM EGF4-6 expressed in E. coli was detected by KPTT kit. Results The expressed product with biological activity contained about 12% of sometic protein. Conclusion The study provides conditions for the further development of biological product for clinical diagnosis and treatment.

关 键 词：血栓调节蛋白 EGF4-6 克隆 表达 鉴定 TM活性蛋白 

分 类 号：R394.8[医药卫生—医学遗传学] Q78[医药卫生—基础医学]