多管藻中R-藻蓝蛋白能量传递途径及动力学  

STUDIES ON THE ENERGY TRANSFER PROCESSES AND DYNAMICS IN R-PHYCOCYANIN FROM Polysiphonia urceolata

作　　者：张建平[1] 谢洁[1] 赵井泉[1] 彭程航[2] 郑锡光[3] 赵福利[3] 张景民[3] 汪河洲[3] 

机构地区：[1]中国科学院化学研究所,北京100101 [2]中国科学院生物物理研究所,北京100101 [3]中山大学激光光谱学国家重点实验室,广东广州510275

出　　处：《生物物理学报》2001年第4期767-772,共6页Acta Biophysica Sinica

基　　金：国家自然科学基金资助 (39870222)

摘　　要：以蛋白亚基复性技术和皮秒级时间分辨荧光光谱 ,研究海洋红藻多管藻中R -藻蓝蛋白(R -PC)单体和三聚体内能量传递过程。利用亚基复性技术对分离后的β亚基复性,以R -藻蓝蛋白单体和β亚基之间的差谱获得α亚基的吸收光谱。皮秒级时间分辨三维谱图(时间、波长和强度)直观地显示出藻红胆素发色团向藻蓝胆素发色团的能量传递;根据时间分辨测量结果的组份解析,对R藻蓝蛋白单体和三聚体内能量传递途径和相关传递参数进行了指认和讨论;对观察到的单体与三聚体能量传递组份特性的差别提出了解释。与C -藻蓝蛋白光谱对比,RThe energy transfer processes in the monomeric and trimeric R- phycocyanin (R-PC) from Polysiphonia urceolata were studied by the picosecond time-resolved spectra and renaturation technique of the protein subunit. The spectral data were obtained directly for the renaturated β subunit of the R-PC and those of the α subunit were considered to be the spectral differences between the spectra of the β subunit and those of R-PC. The three dimensional spectra(time,wavelength and intensity) clearly showed the energy transfer process between the phocoerythrobilin(PEB) and phococyanobilin(PCB) chromophores in R-PC. Based on the componential analyses of the time-resolved spectra, the energy transfer pathways and the kinetic parameters, as well as the difference between the energy transfer features in monomers and the trimers, were discussed and explained in detail. Furthermore, it is indicated that the special chromophore composition is responsible for higher efficiency for light capture and energy transfer in R-PC than in C-PC.

关 键 词：R-藻蓝蛋白 亚基复性 稳态 时间分辨光谱 能量传递动力学 多管藻 捕光天线 

分 类 号：Q949.29[生物学—植物学] Q632