PR-1a蛋白基因的克隆及序列分析  

Cloning and Sequencing of PR-1a Protein Gene

作　　者：陈茹梅[1] 李汝刚[2] 伍宁丰[2] 杨芳 孙盈盈[2] 

机构地区：[1]中国农业大学植物病理系病毒室,北京100094 [2]中国农业科学院生物技术研究所分子室,北京100081 [3]河南省南阳市农技站,河南南阳473053

出　　处：《农业生物技术学报》2001年第4期319-321,共3页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金资助项目;批准号:39700094

摘　　要：以珊西烟草（Ｎｉｃｏｔｉｎａ ｔａｂａｃｕｍ ｃｖ．Ｘａｎｔｈｉ－ｎｃ）的基因组 ＤＮＡ为模板，通过合成一对特异性引物，利用多聚酶链式反应（ＰＣＲ）扩增获得ＰＲ－ｌａ蛋白基因。将其克隆到Ｅ． ｃｏｌｉ质粒上进行序列分析。结果表明：该基因由５０４个碱基组成，编码１６８个氨基酸。与已发表序列相比较，核苷酸序列及推导出的氨基酸序列的同源性分别为９９．９％和１００％。The entire coding sequence of PR-la protein gene was amplified by means of PCR using genomic DNA of Nicotina tab- acum cv. Xanthi-nc as template and a pair of specific oligonucleotides at 5'- and 3'-end as primers, and cloned into E. coil vector. DNA sequence was determined, which consists of 504 bp and encodes 168 amino acid residues deduced from DNA sequence. Comparison with previously published sequence showed 99.99% homologies in nucleotide sequence and 100% in amino acid sequence respectively.

关 键 词：PR-1a蛋白 基因克隆 DNA序列分析 病程相关蛋白 植物 马铃薯 抗病性 

分 类 号：S332.2[农业科学—作物遗传育种] S188[农业科学—农艺学]