团头鲂和广东鲂生长激素cDNA的分子克隆和序列分析  

Molecular Cloning and Sequence Anslysis of Growth Hormone cDNAs from Two Species of Gurnards

作　　者：劳海华[1] 白俊杰[1] 叶星[1] 李英华[1] 罗建仁[1] 

机构地区：[1]中国水产科学研究院珠江水产研究所,农业部热带亚热带鱼类选育与养殖重点开放实验室,广东广州510380

出　　处：《农业生物技术学报》2001年第4期346-349,共4页Journal of Agricultural Biotechnology

基　　金：农业部"九五"重点渔业科技资助项目(渔95-B-96-02-08-02);中国水产科学研究院重点基金资助项目(99-08-02

摘　　要：从团头鲂（Ｍｅｇａｌｏｂｒａｍａ ａｍｂｌｙｃｅｐｈａｌａ）和广东鲂（Ｍｅｇａｌｏｂｒａｍａ　ｈｏｆｆｍａｎｎｉ）的脑垂体组织提取总ＲＮＡ，采用３’ＲＡＣＥ－ＰＣＲ的方法，从中扩增出编码两种鲂生长激素（ｇｒｏｗｔｈ　ｈｏｒｍｏｎｅ，ＧＨ）成熟肽的ｃＤＮＡ序列，克隆到ｐＧＥＭ－Ｔ ＥａｓｙＶｅｃｔｏｒ载体上。克隆的团头鲂和广东鲂ＧＨｃＤＮＡ均包括编码１８８个氨基酸残基的ＧＨ成熟肽序列和３’端的非翻译区，但不含信号肽序列和５’端非编码区。序列分析结果表明，团头鲂和广东鲂ＧＨ的碱基序列和推测的氨基酸序列的同源性分别为９９％和１００％。Total RNAs was isolated from the pituitary of Megalobrama hoffmanni and Megalobrama amblycephala. The cDNAs encoding two species of gurnards GH mature peptides together with 3' end untranslated regions were amplified by 3'RACE-PCR strategy using isolated total RNAs as template. The amplified cDNA fragments were inserted into pGEM-T Easy Vector. The cloned cDNAs in- duded no signal peptides sequence. The GH mature peptides of both gurnards were composed of 188 amino acid residues. The consistecy of the two gurnards nucleotide sequences of mature GH peptides and deduced amino acid sequences were 99% and 100% respecfively.

关 键 词：团头鲂 广东鲂 GH 分子克隆 序列分析 生长激素 

分 类 号：S961.6[农业科学—水产养殖] S963.732[农业科学—水产科学]