红藻氨酸癫痫大鼠海马GFAP基因调控蛋白表达的变化  

EXPRESSION OF REGULATION PROTEIN OF GLIAL FIBRILLARY ACIDIC PROTEIN GENE IN HIPPOCAMPUS OF EPILEPTIC RATS INDUCED BY KAINIC ACID

作　　者：姜春玲[1] 黄敏[2] 安晓飞[1] 张万琴[1] 

机构地区：[1]大连医科大学生理教研室,辽宁大连116027 [2]大连医科大学微生物教研室,辽宁大连116027

出　　处：《中国应用生理学杂志》2001年第4期360-362,共3页Chinese Journal of Applied Physiology

摘　　要：目的和方法 :用Southwestern印迹从红藻氨酸 (KA)癫痫大鼠海马结构中筛选调控胶质原纤维酸性蛋白 (GFAP)基因表达的DNA结合蛋白 ;并观察其在海马内表达变化的规律 ,旨在从基因调控水平深入探讨癫痫反复发作形成的神经病理学机制。结果 :Southwestern印迹的实验显示海马结构内有两种调控GFAP基因表达的序列特异的DNA结合蛋白 ,分子量分别为 39kDa和 35 .5kDa ;KA后 1d ,两种调控蛋白的表达即开始增加 ,5～ 7d时表达显著增加 ,3周时表达最多 ,3个月时表达仍很高。结论 :KA通过上调调控GFAP基因表达的转录因子 ,使海马GFAP过量表达 。Aim and Methods: The DNA binding protein from hippocampus of epileptic rats was investigated by southwestern blot,which regulated the expression of glial fibrillary acidic protein (GFAP) gene. Its expression pattern was also studied. Results: Southwestern blot showed that there were two specific DNA binding proteins that regulated the expression of GFAP gene,and their molecular weight were 39 kDa and 35.5 kDa,respcetively. The expression of the DNA binding proteins was markedly increased in hippocampus of kainic acid (KA)+NS group at 5 d,reached the peak at 3 w,lasted for 3 m observed. Conclusion: The results above suggest that KA may increase the expression of GFAP in hippocampus by upregulating the transcription factor that regulated the expression of GFAP gene,which may participate in the formation of epileptic seizures.

关 键 词：癫痫反复发作 海马 GFAP基因 DNA结合蛋白 调控 

分 类 号：R742.102[医药卫生—神经病学与精神病学]