肾综合征出血热疫苗候选毒株A16株的分子特性  

Molecular Characteristics of the Seed Virus of HFRS Candidate Vaccine A16 Strain

作　　者：姚智慧[1] 董关木[1] 俞永新[1] 贾克丽[2] 严玉辰[2] 

机构地区：[1]中国药品生物制品检定所,北京100050 [2]中国预防医学科学院流行病学微生物学研究所,北京102206

出　　处：《中国病毒学》2001年第4期315-320,共6页Virologica Sinica

摘　　要：为研究肾综合征出血热疫苗候选毒株A16株的分子基础 ,应用RT -PCR方法扩增并测定了A16株的M和S片段的序列 ,结果A16株M片段的全基因序列共 36 15个核苷酸 ,编码 1133个氨基酸。S片段的全基因序列共1770个核苷酸 ,编码 4 2 9个氨基酸。A16株M片段核苷酸全基因序列及其推导的氨基酸与HTN型毒株同源率为75 .5 %～ 90 .3%和 85 .9%～ 97.1% ,即A16与HTN同型毒株间的差异高达 9.7%～ 2 4 .3%和 2 .9%～ 14 .1% ,而与SEO型的同源率比较 ,核苷酸和氨基酸分别仅为 70 .2 %～ 70 .8%和 73.4 %～ 76 .7%。S片段的序列同源率与M片段的结果相类似 ,即核苷酸和氨基酸的同源率与HTN型毒株分别为 76 .0 %～ 90 %和 92 .1%～ 97.7% ,与SEO型为6 7.0 %～ 6 7.7%和 81.7%～ 82 .6 %。结果显示 ,A16株虽然属于HTN型 。For further studying on the molecular characteristics of A16 strain,which is the seed virus of HFRS candidate vaccine,the M and S segments of A16 strain were amplified by RT PCR and then sequenced respectively.Sequence assay showed that Msegment of A16 strain contained 3615 nucleotides,encoding 1133 amino acids,and S segment of A16 strain contained 1 770 nucleotides,encoding 429 amino acids.The homology of M segment of A16 strain's nucleotide sequences and induced amino acid sequences with that of other HTN type virus varied from 75.7% to 90.3% and 85.9% to 97.1%respectively.Namely,the mutation of M segment of A16 strain's nucleotide sequence and amino acid sequence with that of other HTN type virus varied from 9.7% to 24.3% and 2.9% to 14.1% respectivedly.Like M segment,the homology of S segment of A16 strain's nucleotide sequences and induced amino acid sequences with that of other HTN type virus varied from 76.0% to 90.0% and 92.1% to 97.7%,and 67.0% to 67.7% and 81.7% to 82.6% with that of SEO type virus respectively.The result showed that A16 strain was one subtype strain of HTN type virus.

关 键 词：肾综合征出血热疫苗 A16株 S片段 序列分析 系统发生树 亚型 M片断 

分 类 号：R392[医药卫生—免疫学]