番木瓜环斑病毒畸叶株系的CP基因克隆和序列分析  

Cloning and Sequencing of Coat Protein Geng of Malformed Leaf Strain of Papaya Ringspot Virus

作　　者：贺国安[1] 肖火根[1] 张曙光[1] 李华平[1] 范怀忠[1] 

机构地区：[1]华南农业大学植物病毒研究室,广东广州510642

出　　处：《中国病毒学》2001年第4期369-372,共4页Virologica Sinica

基　　金：农业部重点项目"高新技术与基础研究"(4 2 0 0 -C980 0 4);广东省重点科技攻关项目 (99M0 15 0 4G)

摘　　要：采用RT PCR方法合成了本研究室保存的番木瓜畸叶病毒 (PMaLV)的外壳蛋白 (CP)基因 ,将其CP基因克隆进Promega公司的pGEM -TandpGEM -TEasyVectorSystem(简称T -载体 ) ,并进行了序列分析。结果表明 ,PMaLVCP基因核苷酸序列全长为 86 1nt,推导其编码 2 87个氨基酸。与番木瓜环斑病毒 (PRSV)美国夏威夷HA株系和澳大利亚W株系的CP基因相比 ,在第 6 6nt处开始连续缺失 3个核苷酸。与PRSV的华南Ys、Sm和G株系以及夏威夷的HA和澳大利亚的W株系相比 ,其CP基因序列同源率分别为 96 %、98%、95 %、89%和 89%。其推导的氨基酸序列同源率分别为 98%、97%、97%、96 %和 95 %。此结果表明 ,PMaLV属于PRSV的一个株系 ,不是一种新病毒。因此 ,我们称其为番木瓜环斑病毒畸叶株系 (ML株系 )。The coat protein(CP)gene of an isolate of papaya malformed leaf virus(PMaLV),preserved in Plant Virology Laboratory of South China Agricultural University by Luo Xuehai,was synthesized by reverse transcription and polymerase chain reaction(RT PCR) and cloned into pGEM T and pGEM T Easy Vector System(T vector).The full length of the CP gene was sequenced,and it shown that the CP gene was 861 nucleotides(nt) in length,with 3nt deletion beginning from 66nt as compared with that of Hawaii strain(HA) and Australian strain W of papaya ringspot virus(PRSV).Compared with Ys(yellow ringspot strain)?Sm(severe mosaic strain)?G(Hainan isolate)?HA and W strains of PRSV,the CP gene sequence of PMaLV shared 96%?98%?95%?89% and 89% nucleotide sequence identity respectively.The coat protein encoded by CP gene of PMaLV consisted of 287 amino acids residues,and shared 98%?97%?97%?96% and 95% amino acid identity with those of the five strains respectively.This results of sequence analysis shown conclusively that PMaLV is a strain of PRSV,not a new virus,and we termed it malformed leaf strain(ML)of Papaya ringspot virus.

关 键 词：番木瓜畸叶病毒 番木瓜环斑病毒 番木瓜环斑病毒畸叶株系 CP基因克隆 序列分析 

分 类 号：S432.41[农业科学—植物病理学] S436.67[农业科学—农业昆虫与害虫防治]