高对映选择性环氧化物水解酶产生菌的筛选及特性研究  

ISOLATION AND CHARACTERIZATION OF AN EPOXIDE HYDROLASE PRODUCER FOR ENANTIOSELEC TIVE HYDROLYSIS OF (R,S)-PHENYL GLYCIDYL ETHER

作　　者：唐燕发[1] 许建和[1] 武慧渊[1] 叶勤[1] Schulze Birgit 

机构地区：[1]华东理工大学生物反应器工程国家重点实验室生物催化研究室,上海200237 [2]DSM Research,P.O.Box18

出　　处：《微生物学通报》2001年第5期14-17,共4页Microbiology China

基　　金：荷兰DSM公司资助项目 (No.ACER 980 8 0 1 2 6)

摘　　要：从土壤中分离的芽孢杆菌BacillusmegateriumECU1 0 0 1所产环氧化物水解酶能高对映选择性水解缩水甘油苯基醚 (对映选择率E值可达 47 8) ,当转化率为 5 5 9%时 ,剩余的 (S) 缩水甘油苯基醚的光学纯度 (对映体过量值 ,ee)可达 99 5 % ;当底物浓度提高到 60mmol/L时 ,光学纯 (S) 缩水甘油苯基醚的收率达到 2 5 6%A bacterial strain (ECU1001) was isolated from soil and identified to be Bacillus megaterium.The strain shows an activity of epoxide hydrolase that catalyzes the asymmetric hydrolysis of phenyl glycidyl ether,yielding (S)-epoxide and (R)-diol with high enantioselectivity (E=47.8).When the racemic substrate was enzymaticlly hydrolyzed to 55.9% conversion,the enantiomeric excess (ee) of recovered (S)-epoxide was 99.5%.The yield of (S)-phenyl glycidyl ether could reach 25.6% when concentration of the substrate was increased to 60 mmol/L.

关 键 词：缩水甘油苯基醚 手性环氧化物 巨大芽孢杆菌 环氧化物水解酶 菌株筛选 对映选择性 水解 

分 类 号：Q939.99[生物学—微生物学]