p16基因转染对肺腺癌细胞系Anip973和AGZY83-a的生长抑制作用  

Growth inhibition of transfection of p16 gene to lung adenocarcinoma cell lines Anip973 and AGZY83-a

作　　者：王柏秋[1] 闫承慧[1] 高慧[1] 吴焱[1] 王琦[1] 黄承滨[1] 傅松滨[1] 李璞[1] 

机构地区：[1]哈尔滨医科大学遗传学教研室,150086

出　　处：《中国肺癌杂志》2001年第6期444-447,共4页Chinese Journal of Lung Cancer

基　　金：黑龙江省自然科学基金 (Q98 9);黑龙江省科学技术计划攻关项目 (G99C2 0 6 1 )资助

摘　　要：目的　探讨肿瘤抑制基因p1 6对肺腺癌细胞生长的抑制作用。方法　应用FuGene转染方法将p1 6基因的表达质粒转入一对分别具高、低转移能力的肺腺癌细胞系Anip973和AGZY83 a中。对p1 6蛋白过表达的细胞系进行了细胞生长曲线、克隆形成率、原位末端标记分析和流式细胞术分析。结果　p1 6基因的过表达只能使AGZY83 a和Anip973的G1期细胞比例提高 ,但细胞生长曲线、克隆形成率均未发生改变 ,原位末端标记分析也未发现凋亡信号。结论　本实验结果提示p1 6基因在AGZY83 a和Anip973中的过表达不能抑制它们的生长。Objective To investigate the suppression effect of tumor suppressor gene p16 on lung adenocarcinoma cell lines. Methods p16 expression vector was transfected into high metastasis lung carcinoma cell line, Anip973, and low metastasis lung cancer cell line, AGZY83 a. The suppression effects of p16 gene on cell growth was detected by cell growth curve, cloning efficiency assay, flow cytometry analysis and TUNEL technique. Results Overexpression of p16 gene in Anip973 and AGZY83 a could only lead the cells to arrest at G1 phase, but had no effects on cell growth curves, colony formation rate and apoptosis. Conclusion Overexpression of p16 in Anip973 and AGZY83 a can not suppress the growth of the tumor cells.

关 键 词：P16基因 转染 基因治疗 肺腺癌 

分 类 号：R734.2[医药卫生—肿瘤]