TGF-β_1对角膜上皮移植后CD_4^+、CD_8^+、CD_(25)^+和CD_(71)^+的影响  

Effect of TGF- β_1 on expression of CD_4^+ , CD_8^+, CD_(25)^+ and CD_(71)^+ after corneal epithelium transplantation

作　　者：吴静[1] 徐锦堂[1] 陈剑[1] 赵松滨[1] 

机构地区：[1]中国广州暨南大学医学院眼科,510632

出　　处：《美中国际眼科杂志》2001年第3期7-9,共3页

基　　金：中国国家自然科学基金(39870855)项目;广东省重点科技攻关项目(2KM05203S)

摘　　要：目的 为有效控制角膜移植术后排斥反应的发生,提高角膜移植成功率,我们在小鼠(BALB/c)角膜上皮移植术后应用TGF-β_1,并观察其对外周血淋巴细胞亚群CD_4^+、CD_8^+、CD_(25)^+和CD_(71)^+活化的影响,为今后临床应用TGF-β_1抑制角膜移植术后免疫排斥反应奠定基础。方法 采用CD_4^+、CD_8^+、CD_(25)^+和CD_(71)^+免疫荧光标记及流式细胞仪检测技术,分别对设立的阴性对照组、阳性对照组、同基因组、异体角膜上皮组及TGF-β_1治疗组的BALB/c小鼠角膜上皮移植术后12d的外周血中CD_4^+4CD_(25)^+、CD_8^+CD_(25)^+、CD_4^+CD_(71)^+和CD_8^+CD_(71)^+的表达进行分析。结果 BALB/c小鼠角膜上皮移植术后12d的外周血中CD_(25)^+CD_4^+、CD_(25)^+CD_8^+、CD_(71)^+CD_4^+和CD_(71)^+CD_8^+双阳性的T淋巴细胞均有显著升高;术后经TGF-β_1治疗后,上述细胞的数量明显受到抑制。结论 角膜上皮移植术后应用TGF-β_1可抑制特异性抗原介导的,以及非特异性炎症诱导的移植排斥反应。Objective To study the effect of TGF- β1 on change of CD+4 , CD+8, CD+25, and CD+71T cell population induced by corneal epithelium transplantation. Methods The expression of CD+4 , CD+g, CD+25 and CD+71 in peripheral T cells of BALB/c mice was identified at the 12 days after corneal epithelium transplantation by using indirect immune fluorescence flow cytometry analysis system. Results The expression of CD+4 CD+25, CD+8 CD+25, CD+4CD+71 and CD+8CD+71 in peripheral T cells of BALB/c mice was significantly increased at 12 days after corneal epithelium transplantation. However, the expression of CD+25CD+4 , CD+25CD+8, CD+71CD+4 and CD+71CD+8 was significantly decreased while TGF- β1 was added after the surgery. Conclusion TGF- β1 can significantly inhibit the specific immune rejection mediated both by grafting and nonspecific inflammatory infiltration.

关 键 词：转化生长因子-Β CD25^+ CD71^+ 角膜上皮移植 免疫排斥反应 

分 类 号：R779.65[医药卫生—眼科] R363.27[医药卫生—临床医学]