儿茶素类抗乙型肝炎病毒的效果观察  

Studies on the effects of tea catechins against hepatitis B virus infection

作　　者：李建祥[1] 周立人[1] 章瑜[1] 陈跃进[1] 

机构地区：[1]江苏省苏州大学核医学院卫生毒理学教研室,215007

出　　处：《中华预防医学杂志》2001年第6期404-407,共4页Chinese Journal of Preventive Medicine

摘　　要：目的　观察儿茶素类抗乙肝病毒和保肝降酶作用。方法　用 1日龄北京雏鸭制备鸭乙型肝炎病毒 (DHBV)模型 ,设儿茶素类给药组 (6 0、12 0和 2 40mg/kg)、阳性药物对照组 (Are AMP)、模型组和阴性对照组。取血清用斑点杂交法 (dig标记探针 )测DHBV·DNA ,用单抗夹心ELISA法测定DHBsAg ;检测丙氨酸转氨酶 (ALT)、天冬氨酸转氨酶 (AST)活性观察肝功能变化 ,并作肝组织病理切片观察形态学改变。结果　儿茶素类各给药组鸭血清DHBV·DNA和DHBsAg含量均有降低 ,与给药前和模型组相比差异有显著性 ;给药 2 5d及停药后 14d ,鸭血清ALT与AST活性均降低 ;给药后的肝组织病理变化有明显改善。结论　儿茶素类有抗乙肝病毒、降低转氨酶活性。Objective To examine the effects of tea catechins against hepatitis B virus and their roles in protecting liver damage Methods A duck hepatitis B virus infection model was established by using one day old Beijing ducklings The infected ducklings were divided into six groups: three treatment groups of different dosages (60, 120 and 240 mg/kg of tea catechins p o , 93 7% total catechins and 48 7% EGCG), positive control group (Are AMP, i v ), model group (water, p o ) and negative control group (no DHBV, no catechins) Serum DHBV DNA was tested by dot blot hybridization (marked with digogenin), DHBsAg was assayed by ELISA The morphologic changes of liver and the activities of ALT and AST were also measured Results Levels of DHBsAg and DHBV DNA in duck serum were significantly decreased in all the three catechin treated groups ( P <0 05, P <0 01) Activities of ALT and AST were also significantly decreased after treatment for 25 days and 14 days after the end of the treatment ( P <0 05, P <0 01) Results of liver pathological examinations also provided positive evidence Conclusions The protective effects of tea catechins against hepatitis B virus infection were significant It protected the liver functions and reduced the pathological changes of the liver tissue

关 键 词：儿茶素 DNA 乙型肝炎病毒 抗肝炎病毒 

分 类 号：R965[医药卫生—药理学]