赤魟尾刺cDNA表达文库的构建  

CONSTRUCTION OF cDNA EXPRESSION LIBRARY FROM THE CAUDAL SPINE OF DASYATIS AKAJEI

作　　者：涂洪斌[1] 卫剑文[1] 姜孝玉[1] 陈慧萍[1] 钟肖芬[1] 吴文言[1] 徐安龙[1] 

机构地区：[1]中山大学生命科学学院国家高新技术计划海洋生物功能基因组开放实验室,广东广州510275

出　　处：《药学学报》2001年第12期906-909,共4页Acta Pharmaceutica Sinica

基　　金：国家高新技术海洋领域 86 3项目 ( 819 0 6 0 6 ) ;国家自然科学基金委重点项目 ( 6 9935 0 2 0 )

摘　　要：目的　以赤尾刺为出发材料 ,构建以真核表达载体pcDNA 3 0为基础的赤尾刺cDNA表达文库。方法　应用SMARTTMcDNALibraryConstruction技术和初步生物信息学分析等方法。结果　通过对亚文库克隆的序列测定和初步生物信息学分析 ,已获得了 5 6个赤新EST序列 ,其中已确定的全长cDNA克隆有 2 4个 ,包括IPL肿瘤抑制因子、亲环素cyclophilinA、硒蛋白selenoproteinW、半胱氨酸蛋白酶抑制剂cystatinA、tyrosyl tRNAsynthetase和calcineurin类似物蛋白等。结论　这些基因在赤中绝大多数均为首次发现。赤尾刺cDNA表达文库的成功构建 。AIM A cDNA expressional library from the caudal spine of Dasyatis akajei was constructed into pcDNA 3 0 eukaryotical expression plasmid. METHODS SMART TM protocol was used for cDNA Library Construction and bioinformatics analysis was carried out. RESULTS Fifty six novel EST clones were obtained from the library, in which there were 24 full length clones, including IPL tumor suppressor gene, cyclophilin A, selenoprotein W, cystatin A, tryosyl tRNA synthetase and calcineurin homologous protein. CONCLUSION These genes were mostly first reported in Dasyatis akajei. Further studies may partly explain the molecular mechanisms by which the caudal spine of Dasyatis akajei were often used in the treatment of inflammation, parasitosis and cancer in the traditional Chinese medicine.

关 键 词：赤魟 尾刺 CDNA表达文库 海洋药物 中药 

分 类 号：R282.770.5[医药卫生—中药学]