非水毛细管电泳分离六种抗精神病药物  

ANALYSIS OF SIX ANTIPSYCHOTIC DRUGS BY NONAQUEOUS CAPILLARY ELECTROPHORESIS

作　　者：胡琴[1] 田颂九[2] 许鸣镝[2] 

机构地区：[1]北京市药品检验所,北京100035 [2]中国药品生物制品检定所,北京100050

出　　处：《药学学报》2001年第12期928-931,共4页Acta Pharmaceutica Sinica

摘　　要：目的　用非水毛细管电泳法同时分离分析 6种抗精神病药物。方法　采用未涂层石英毛细管柱 (75 μm× 5 7cm ,有效分离长度为 5 0cm) ,以含 1 0 %冰醋酸的 15 0mmol·L- 1 乙酸钠的甲醇 乙腈 (75∶2 5 )溶液为运行缓冲液 ,压力进样 2s,运行电压为 2 0kV ,检测波长为 2 5 4nm。结果　分离效率高 ,精密度较好 ,所选浓度范围内线性关系良好 ,γ值均大于 0 999。结论　方法简便可靠 ,分析时间短 ,可同时分离分析AIM To develop a nonaqueous capillary electrophoresis method for simultaneous determination of six antipsychotic drugs. METHODS The separation was performed on a fused silica capillary of 75 μm ID×57 cm (50 cm of effect length), the running buffer was methanol acetonitrile ( 75∶25 ) containing 150 mmol·L -1 ammonium acetate and 1 0% glacial acetic acid. Samples were solved in the buffer containing 15 mmol·L -1 ammonium acetate and injected by pressure mode. The applied voltage was 20 kV and the detection wavelength was set at 254 nm. RESULTS The method was validated in terms of reproducibility, linearity (γ>0 999 for all analytes), limits of quatitation and detection. The paper also discussed the buffer composition, applied voltage and temperature which influenced the separation. CONCLUSION The nonaqueous capillary electrophoresis method is effective, simple, fast and reliable. The six antipsychotic drugs which have similar structrure can be separated by this method at the same time, but only five of them can be separated by capillary zone electrophoresis which was based on the aqueous buffer. At the same concentration of buffer in nonaqueous capillary electrophoresis and capillary zone electrophoresis, the former would acquire much lower current and less Joule heat.

关 键 词：非水毛细管电泳 抗精神病药物 氯普噻吨 奥氮平 哌泊噻嗪 奋乃静 

分 类 号：R971.4[医药卫生—药品] R927[医药卫生—药学]