福氏2a志贺菌痢暴发的RAPD分析  

RAPD ANALYSIS IN AN OUTBREAK OF S.FLEXNERI 2A IN JIANGMEN

作　　者：郝加虎[1] 叶冬青[1] 王红[2] 钟文龙[3] 夏桂枝[2] 吴爱军[2] 俞守义[2] 

机构地区：[1]安徽医科大学公共卫生学院流行病学教研室,合肥230032 [2]第一军医大学流行病学教研室 [3]广东省江门市卫生防疫站

出　　处：《中国人兽共患病杂志》2001年第6期26-28,共3页Chinese Journal of Zoonoses

摘　　要：目的　探讨江门一起菌痢暴发的特异传染源。方法　对 8株福氏 2a志贺菌进行RAPD分析。结果　此次菌痢暴发期间分离的 8株福氏 2a志贺菌具有两种不同的RAPD特征 (RTⅠ、RTⅡ型 ) ,其中 2株病例密切接触者分离株J9810 1(RTⅡ型 )和J982 6(RTⅠ型 )分属不同克隆。结论　本次暴发偶合有部分散发病例。相对于质粒分析而言 ,RAPD分析具有更高的分辨率 ,它提供的遗传学信息更为丰富、直接。Aim To explore the special source of shigellosis infection outbreak in Jiangmen.Methods RAPD analysis was carried out on 8 strains of S.flexneri 2a.Results There were two different RAPD patterns in 8 of S.flexneri 2a (RTⅠ?RTⅡ),among them,two contact strains,J98101 and J9826,belong to different clones,RTⅠand RTⅡ,suggesting that some patients,who were included in the outbreak event by coincidence,were not true outbreak cases.Conclusion There were some sporadic cases which were included in the outbreak by chronological and special coincidence.RAPD analysis had high distinguish ability and could provide more direct,rich and precise genetic information than plasmid profile analysis.

关 键 词：福氏2A志贺菌 RAPD 细菌性痢疾 

分 类 号：R378.25[医药卫生—病原生物学] R516.403[医药卫生—基础医学]