结直肠癌组织中MTS1/p16基因表达检测的意义  

DETECTION OF MTS1/p16 GENE EXPRESSION IN COLORECTAL CANCER BY RT-PCR

作　　者：王春雷[1] 黄志强[1] 王燕生[1] 刘海玲[2] 

机构地区：[1]解放军总医院,北京100853 [2]中国医学科学院

出　　处：《解放军医学杂志》2001年第12期902-903,共2页Medical Journal of Chinese People's Liberation Army

摘　　要：为探讨人结直肠癌组织中MTS1/ p16基因的缺失情况 ,利用逆转录聚合酶链反应来检测取自结直肠癌患者的 6 0例手术切除组织标本中MTS1/ p16基因的等位缺失情况 ,其中结直肠癌组织 35例 ,癌旁组织 15例及结直肠正常粘膜组织 10例。结果显示 ,17 14 % ( 6 / 35 )的结直肠癌组织标本出现MTS1/p16基因的等位缺失 ,而癌旁组织及结直肠正常粘膜均未出现MTS1/ p16基因的缺失。提示MTS1/ p16基因的缺失在结直肠癌进展过程中可能起着一定的促进作用。To investigate the deletion of MTS1/p16 gene in human colorectal carcinoma,the homozygous deletion of MTS1/p16 gene was checked by reverse transcriptase polymerase chain reaction from 60 patients with colorectal carcinoma.The specimens consisted of 35 colorectal carcinoma,15 tumor-adjacent tissues and 10 normal colorectal mucosa. It was found that 17 14% (6/35) colorectal carcinoma specimens showed homozygous deletion of MTS1/p16 gene.No deletion was detected in tumor-adjacent tissue and normal colorectal mucosa.Deletion of MTS/p16 gene may play a role in the progression of colorectal carcinoma.

关 键 词：MTS1/p16基因 直肠肿瘤 基因缺失 聚合酶链反应 直肠癌 结肠癌 结肠肿瘤 

分 类 号：R735.35[医药卫生—肿瘤] R735.37[医药卫生—临床医学]