高压氧暴露后急性脑缺血动物血细胞形态改变的实验研究  

Morphologic Changes of Blood Cells after Hyperbaric Oxygen Exposure in Animals with Cerebral Ischemia

作　　者：蔺世龙[1] 吴学明[2] 黄荣国 辛佩珠[1] 刘景昌[1] 骆秉铨 

机构地区：[1]上海海军医学研究所,200433 [2]无锡市第三人民医院 [3]徐州心血管病研究所

出　　处：《中国微循环》2001年第4期268-270,共3页Journal of Chinese Microcirculation

摘　　要：目的研究动物急性缺血性脑损伤时血细胞形态改变及高压氧暴露后的治疗作用。方法阻断沙鼠双侧颈动脉30min,造成急性脑缺血损伤。然后在250kPa高压氧下暴露60min,用布氏和OPTON显微镜观测了动物活血细胞形态变化。结果动物急性缺血性脑损伤时 ,红细胞聚集明显 ,血中有较多的棘型红细胞 ,红细胞正常凹面消失 ,影子红细胞和红细胞碎片增多。白细胞数量增加 ,伪足形成 ,细胞内碎片等形成较大的血栓块。但脑缺血损伤动物经250kPa高压氧暴露后上述改变明显减轻。结论高压氧对急性缺血性脑损伤动物的白细胞、血小板活化具有调整作用 ,并具有保护红细胞的正常功能 。Objective To study the morphologic changes of blood cells and the therapeutic effects of hyperbaric oxygen (HBO) in animals with cerebral ischemia. Methods Carotids were blocked for 30min, so that cerebral ischemia occurred in gerbils. The animals were exposed to HBO at 250kPa for 60min. The shape of the blood cells was observed through microscope.Results In animals with cerebral ischemic injury, there were many spinous red blood cells (RBC). RBC aggregated obviously and normal RBC disc concave disappeared. Shadow RBC and its fragments increased. White blood cell (WBC) number increased and the granulocorpuscles in WBC were very active. WBC and platelet pseudopod formed. Blood cells aggregated with fat droplet and others to form thrombosis. However,above changes were obviously reduced after HBO exposure in animals with cerebral ischemia. Conclusions HBO has the role of regulating activation of WBC and platelets during cerebral ischemic injury in animals. Meanwhile, HBO may protect RBC normal function and delay the formation of thrombus during cerebral injury.

关 键 词：细胞流变性 脑缺血 高压氧 血栓 微循环 

分 类 号：R743.31[医药卫生—神经病学与精神病学]