应用RT-PCR方法检测水稻植株和介体昆虫体内的水稻齿叶矮缩病毒  

RT-PCR DETECTON OF Rice ragged stunt virus IN RICE PLANT AND INSECT VECTOR

作　　者：雷娟利[1] 吕永平[1] 金登迪[1] 陈声祥[1] 陈剑平[1] 

机构地区：[1]浙江省农业科学院病毒室,杭州310021

出　　处：《植物病理学报》2001年第4期306-309,共4页Acta Phytopathologica Sinica

基　　金：美国洛氏基金 ( RF980 0 1#68) ;浙江省青年科技人才专项基金 ( RC960 4 ) ;浙江省重点实验室专项 ( 0 110 75 5 7)

摘　　要：将生物学接种和 RT- PCR检测水稻植株体内的水稻齿叶矮缩病毒的方法进行了比较 ,发现结果基本趋于一致 ,但总体上 RT- PCR检测阳性率稍高于生物学接种的发病率。将生物学接种检测褐飞虱介体内水稻齿叶矮缩病毒方法与 RT- PCR检测方法进行比较分析 ,发现 RT- PCR方法可以检测单头褐飞虱体内的水稻齿叶矮缩病毒 ,具有很高的准确性和灵敏度。RT PCR was compared with biological method in detecting Rice ragged stunt virus (RRSV) in rice plants. Results indicated that RT PCR method gave corresponding result to that of biological inoculation, but in general, RT PCR method was more sensitive. Compared with detection of the RRSV in insect vector by virus transmission, RT PCR was also more accurate and the RRSV could be detected even in a single vector.

关 键 词：水稻齿叶矮缩病毒 RT-PCR检测 生物学接种 症状 介体昆虫 

分 类 号：S435.111.4[农业科学—农业昆虫与害虫防治] S432.41[农业科学—植物保护]