醇类助溶剂对拟低共熔体系中酶促合成ZAPM反应的影响  

EFFECT OF ALCOHOLS ON ENZYME-CATALYZED SYNTHESIS OF ZAPM IN PSEUDO-EUTECTIC MIXTURES OF SUBSTRATES

作　　者：沈树宝[1] 柴本忠[1] 胡永红[1] 欧阳平凯[1] 

机构地区：[1]南京化工大学制药与生命科学学院,江苏南京210009

出　　处：《化工学报》2001年第12期1109-1112,共4页CIESC Journal

基　　金：国家自然科学基金 (No .2 9976 0 19);江苏省自然科学基金 (No .BK9912 3)资助项目~~

摘　　要：In this work,the effect of alcohols (including alcohols containing single, two, three hydroxyl groups, respectively) on the enzyme- catalyzed synthesis of Z-L-Asp-PheOMe (ZAPM) is investigated by using a new technique of enzymatic catalysis in a heterogeneous pseudo-eutectic solid-to-solid mixture with thermolysin.The experimental result shows that the alcohols containing two hydroxyl groups are the most suitable adjuncts for the studied enzymatic synthesis system, and the yield of ZAPM for 1,4-butanediol as an adjunct reaches 95% at reaction time 2?h, which is better than the results in two-phase systems and other pseudo-eutectic solid-to-solid mixture reported in literature.In this work,the effect of alcohols (including alcohols containing single, two, three hydroxyl groups, respectively) on the enzyme- catalyzed synthesis of Z-L-Asp-PheOMe (ZAPM) is investigated by using a new technique of enzymatic catalysis in a heterogeneous pseudo-eutectic solid-to-solid mixture with thermolysin.The experimental result shows that the alcohols containing two hydroxyl groups are the most suitable adjuncts for the studied enzymatic synthesis system, and the yield of ZAPM for 1,4-butanediol as an adjunct reaches 95% at reaction time 2?h, which is better than the results in two-phase systems and other pseudo-eutectic solid-to-solid mixture reported in literature.

关 键 词：低共熔体系 醇 酶促反应 助溶剂 ZAPM 苄氧羰酰-L-天门冬氨酰-L-苯丙氨酸甲酯 

分 类 号：O625.6[理学—有机化学] O643.31[理学—化学]