胰岛素样生长因子和HBV-DNA整合在肝癌演变过程中作用机制的研究  

Study on the Action Mechanism of Insulin-Like Growth Factors and HBV-DNA Integration During Hepatocarcinogenesis

作　　者：覃汉荣[1] 范子荣[1] 黄纯炽[1] 崔俊[1] 杨惠娟 毕向军[1] 杨冬华[2] 

机构地区：[1]第一军医大学珠江医院消化科,广东广州510282 [2]暨南大学医学院第一附属医院消化科

出　　处：《中国医师杂志》2001年第11期816-820,共5页Journal of Chinese Physician

摘　　要：目的　通过观察胰岛素样生长因子及其受体 (IGFs)和增殖细胞核抗原 (PCNA)在不同肝组织的表达 ,探讨它们与HBV -DNA整合在肝癌发生中的关系 ,为肝癌的发病机制和临床诊疗提供一定的理论依据。方法　肝癌和癌旁肝组织各 32例 ,慢性活动性乙型肝炎 12例 ,胎肝 10例和正常肝组织 8例 ,Southern杂交检测各种肝组织中HBV -DNA整合情况 ,胶体金免疫电镜技术观察不同肝组织中IGFs和PCNA在超微结构水平的分布与表达。结果　 81 3% (2 6/32 )的肝癌和癌旁肝组织为HBV -DNA整合阳性。 83 3% (10 /12 )的慢性活动性乙型肝炎为HBV -DNA整合阳性。所有胎肝组织和正常成人肝组织均无HBV -DNA整合。 87 5 % (2 8/32 )的肝癌和84 4 % (2 7/32 )癌旁肝组织的表达PCNA ;肝癌组织表达胰岛素样生长因子Ⅰ (IGF -Ⅰ )和IFG -Ⅰ受体 (IGF -ⅠR)的阳性率分别为 4 0 6% (13/32 )和 5 6 3% (18/32 ) ;癌旁肝组织表达IGF -Ⅰ和IGF -ⅠR的阳性率分别为5 0 0 % (16/32 )和 65 6% (2 1/32 )。肝癌组织表达胰岛素样生长因子Ⅱ (IGF -Ⅱ )和IGF -Ⅱ受体 (IGF -ⅡR)的阳性率均为 78 1% (2 5 /32 ) ;癌旁肝组织表达IGF -Ⅱ和IGF -ⅡR的阳性率均为 81 3% (2 6/32 )。HBV -DNA整合组中 ,肝癌和癌旁肝组织表达IGF -Ⅱ和IGF -ⅡR的阳性率?Objective To observe the expressions of insulin-like growth factors(IGFs) and proliferating cell nuclear antigen(PCNA) in various kinds of liver tissues,we discussd the relationship among IGFs,PCNA and HBV-DNA integration in the process of hepatocarcinogenesis,provide some theoretical basis for the mechanism of hepatocarcinogenesis and clinical diagnosis and therapy of HCC.Methods 32 cases of HCC,32 cases of paracancerous liver tissues(PLT),12 cases of chronic active hepatitis(CAH),10 cases of fetal liver tissues(FLT) and 8 cases of normal adult liver tissues(NALT) were collected.Southern hybridization was used to detect HBV-DNA integration in various kinds of liver tissues.Then a colloid gold immunoelectron microscopic technique was used to make a systematic study on the expression and ultrastructural location of IGFs and PCNA in different liver tissues.Results The HBV-DNA integration was positively existed in 81 3%(26/32) of HCC and PLT,and 83 3%(10/12) in CAH,but the integration in NALT and FLT was no existed.PCNA was expressed by 87 5%(28/32) of HCC and 84 4%(27/32) of PLT,respectively.The postive rates of IGF-Ⅰ and IGF-ⅠR expressed by HCC were 40 6%(13/32) and 56 3%(18/32),those of IGF-Ⅰand IGF-ⅠR expressed by PLT were 50 0%(16/32) and 65 6%(21/32),respectively.The positive rates of IGF-Ⅱ and IGF-Ⅱ receptor(IGF-ⅡR) expressed by HCC were both 78 1%(25/32),and those expressed by PLT were both 81 3%(26/32).In the integration group of HBV-DNA had higher positive rates of IGF-Ⅱ and IGF-ⅡR,which were expressed by the HCC and PLT groups,than that of non-integration group of HBV-DNA(P<0 005).There were three types of IGFs distribution:inside cytoplasm,perinucleus and in nucleus.No IGFs and its receptor and PCNA were expressed in NALT,but all FLT expressed IGFs and PCNA.Conclusions HBV-DNA integration exists in most of HCC and PLT.The abnormal expression of IGF-Ⅱ gene and IGI-ⅡR gene could play a very important role in the development of HCC.HBV-DNA integration might activa

关 键 词：肝细胞肝癌 胰岛素样生长因子 乙型肝炎病毒 胶体金免疫电镜 

分 类 号：R735.7[医药卫生—肿瘤]