基因新异构体的剪接特征及其生物学意义  

The Splicing Characteristics and Biological Significance of New Isoforms in Novel Full-Length cDNA Cloning

作　　者：彭永德[1] 宋怀东[1] 高国峰[1] 李月彬 黄长辉 胡仁明[1] 韩泽广 陈家伦[1] 

机构地区：[1]上海第二医科大学瑞金医院上海市内分泌研究所,上海200025 [2]国家人类基因组南方研究中心

出　　处：《上海第二医科大学学报》2001年第4期292-295,共4页Acta Universitatis Medicinalis Secondae Shanghai

基　　金：国家自然科学基金资助项目 (39970 370 )

摘　　要：目的探讨基因新异构体的剪接特征及生物学意义。　方法对人肾上腺组织cDNA文库作大规模EST分析及全长cDNA克隆。　结果发现 7条由不同剪接方式形成的新的异构体 ,其中 4个剪接特点不符合经典的gt-ag规律 ,且剪接方式多样 ,人类固醇 5α还原酶跨越两个外显子 ,KIAA0 971的异构体与KIAA0 971比较 ,有两段缺失 ,且造成编码框架移动 ,均按 gt-ag方式。人 17.9kd蛋白与从基因组序列预测的cDNA比较增加一个新外显子 ,人甲状腺激素受体相互作用蛋白 15的第 5号外显子端具有 2个 -ag的接点。　Objective To explore the splicing patterns and biological significance of new isoforms in full-length cDNA cloning. Methods Large_scale analysis of expressed sequence tags (ESTs) from human adrenal cDNA and full-length cDNA cloning were carried out. Results Seven isoforms by different splicing patterns were identified. Four of them did not follow the typical gt_ag pattern. In human steroid 5_alpha_reductase splicing spanned 2 exons. Two sections of deletion were found in the new isoform of KIAA0971 by the gt-ag splicing pattern and ORF was shifted. Comparing with the genomic sequence, a new exon was increased in human 17.9kd protein (a new isoform), and there were two-ag adaptors in the 3' end of exon5 of the human thyroid receptor interactor 15. Conclusion It is suggested that the splicing patterns are diverse and complex in the processing of mRNA in human beings.

关 键 词：全长CDNA克隆 异构体 剪接方式 生物学 

分 类 号：Q78[生物学—分子生物学]