红外能谱仪检测^(13)C-尿素呼气试验诊断幽门螺杆菌感染  

^(13)C-urea Breath Test with Infrared Heterodyne Ratiometry in the Diagnosis of Helicobacter Pylori Infection

作　　者：吴叔明[1] 高琴琰[1] 张德中[1] 萧树东[1] 

机构地区：[1]上海第二医科大学附属仁济医院上海市消化疾病研究所,200001

出　　处：《胃肠病学》2001年第4期206-208,共3页Chinese Journal of Gastroenterology

摘　　要：目的：验证红外能谱仪检测１３Ｃ－尿素呼气试验（ＵＢＴ）诊断幽门螺杆菌（Ｈ．ｐｙｌｏｒｉ）感染的可靠性，并与气体质谱仪检测结果作一比较。方法：对７６例患者以红外能谱仪进行１３Ｃ－ＵＢＴ检测Ｈ． ｐｙｌｏｒｉ感染，Ｈ． ｐｙｌｏｒｉ感染状态由快速尿素酶试验、组织学检查以及细菌培养确定。其中１７例患者的呼气样本分别用红外能谱仪与气体质谱仪进行检测。结果：７６例患者中Ｈ． ｐｙｌｏｒｉ阳性者４３例（５６．６％），红外能谱仪进行１３Ｃ－ＵＢＴ的测定结果为４１例（５３．９％），其敏感性和特异性分别达到９３．０％和９７．０％。气体质谱仪与红外能谱仪的检测数值相差无几。结论：以红外能谱仪进行１３Ｃ－ＵＢＴ检测可靠、准确地诊断Ｈ．ｐｙｌｏｒｉ感染，并具有简单、实用的特点。红外能谱仪与气体质谱仪一样可被广泛应用于Ｈ．ｐｙｌｏｒｉ检测。Background/Aims: To confirm the sensitivity and specificity of 13C-urea breath test (UBT) using infrared heterodyne ratiometry in the diagnosis of Helicobacter pylori (H. Pylori) infection and to com- pare its value with gas-isotope-ratio mass spectrometry. Methods: Seventy-six patients were tested for H. pylori infection using 13C-UBT with infrared heterodyne ratiometry, rapid urease test, histologic ex- amination and culture. 13C-urea breath tests were measured by both infrared heterodyne ratiometry and gas-isotope-ratio mass spectrometry in 17 patients. Results: Forty-three of 76 cases were H.pylori positive (56.6%). The sensitivity and specificity of 13C-UBT with infrared heterodyne ratiometry were 93.0% and 97.0%, respectively. The results measured by two instruments were almost the same. Con- clusions: 13C-UBT using infrared heterodyne ratiometry is as accurate as gas-isotope-ratio mass spec- trometry. UBT using both of these two instruments is highly sensitive and specific in the diagnosis of H.pylori infection.

关 键 词：螺杆菌 幽门呼吸试验 红外能谱仪 消化性溃疡 慢性胃炎 

分 类 号：R573[医药卫生—消化系统]