老年性痴呆大鼠模型建立的研究  

ESTABLISHMENT OF A RAT MODEL WITH ALZHEIMER' S DISEASE

作　　者：李亚明[1] 朱粹青[2] 林水淼[3] 赵伟康[3] 

机构地区：[1]华东医院中医科,上海市200040 [2]复旦大学医学院神经生物国家重点实验室,200032 [3]上海中医药大学老年医学研究所

出　　处：《老年医学与保健》2001年第4期214-217,共4页Geriatrics & Health Care

基　　金：国家自然科学基金重点资助项目;基金编号:39830450

摘　　要：目的研制并建立同时具备老年斑、神经纤维缠结两大病理特征形成过程的AD大鼠模型。方法将D-半乳糖皮下注射和鹅膏蕈酸脑组织定向注射相结合作用于大鼠,分别采用Y-电迷路和免疫组化方法,检测造模大鼠学习记忆和脑组织β-AP含量、磷酸化tau蛋白水平。结果造模大鼠学习记忆成绩下降,脑组织β-AP含量增多,磷酸化tau蛋白水平增高。结论造模大鼠同时具备学习记忆障碍,脑组织β-AP含量增多和磷酸化tau蛋白水平增高及胆碱能功能下降等特征,为目前较为理想的AD模型之一。Objective A rat model with Alzheimer' s Disease(AD)was established that could repeat th e formation of senile pigmented patch and nerve fiber tangle.which were the two pathological c haracteristics of AD.Methods Hypodermic injection of D -galactose and cerebral directive injection of ibotenic acid gave an AD rat model.Y -electric labyrinth was used to test for rat learning and memory functions.Immu nohistochemical methods was used to determine cerebral amyloidβ-peptide(β-AP)content and phosphorylated tau -protein lev el.Results AD rats' learning and memory functions decre ased,cerebralβ-AP contents and phosphorylated tau -protein levels increased.Conclusions The AD model rats had the following ch aracteristics:learning and memory functions were obstructed,c erebralβ-AP contents and phosphorylated tau-protein levels were increased,cholinergic function was decreased.Therefore,this model at present is one of the satisfactory experimental animal model of Alzheimer' s dis-ease.[

关 键 词：D-半乳糖 鹅膏蕈酸 学习记忆 动物模型 老年性痴呆 Β-AP TAU蛋白 

分 类 号：R749.16[医药卫生—神经病学与精神病学] R－332[医药卫生—临床医学]