几种淡水鱼血清免疫复合物的初步研究  

Circulating immune complexes of some fresh water fishes

作　　者：彭宣宪[1] 章跃陵[1] 王三英[1] 

机构地区：[1]厦门大学生命科学学院

出　　处：《中国水产科学》2001年第3期1-4,共4页Journal of Fishery Sciences of China

基　　金：国家自然科学基金资助项目 ( 39770 5 85 ) ;IFS基金资助项目 (A/2 338-2 )

摘　　要：采用SDS -PAGE技术和免疫酶斑点法 (Dot -ELISA) ,对罗非鱼、草鱼、鲫、鳙和鲤 5种淡水鱼的血清免疫复合物 (IC)进行了研究。结果表明 ,鲤科的 4种鱼之间的电泳条带差异较小 ,丽鱼科的罗非鱼与鲤科的 4种鱼之间的电泳条带有差异但较小。 2 %PEG能较好地沉淀 5种鱼血清IC ,且不同鱼血清IC含量有一定的差异。Circulating immune complexes of Tilapia, Ctenopharyngodon idellus, Carassius auratus, Aristichthys nobilis and Cyprinus carpio were characterized by SDS-PAGE and Dot-ELISA. The results show that the circulating immune complexes of the 5 fishes can be isolated by 4% PEG, and the differences between the 4 fishes of Cyprinidae are not very obvious in the bands of electropherogram, and neither does the difference between Tilapia and the 4 Cyprinidae fishes, but there are some obvious difference between these immune complexes, which may be related to the molecular weight of their antibodies.

关 键 词：淡水鱼 免疫复合物 SDS-PAGE 免疫酶斑点法 血清 

分 类 号：S941[农业科学—水产养殖] Q959.4[农业科学—水产科学]