我国野生大豆与栽培大豆AFLP指纹图谱研究  

AFLP Fingerprint Analysis of G.soja and G.max in China

作　　者：田清震[1] 盖钧镒[1] 喻德跃[1] 吕慧能[1] 贾继增[2] 

机构地区：[1]南京农业大学大豆研究所 [2]中国农业科学院品种资源研究所

出　　处：《中国农业科学》2001年第5期480-485,共6页Scientia Agricultura Sinica

基　　金：国家"九五"重点科技项目 ( 960 14 0 10 6)

摘　　要：利用 Mse 和 Eco R 酶切 ,筛选了适宜大豆 AFL P指纹分析的引物组合 ,并利用 17对引物组合 ,建立了我国 92份代表性野生大豆和栽培大豆的 AFL P指纹图谱 ,分析了野生大豆与栽培大豆指纹图谱的特点与差别 ,发现了具有大豆种间特异性的带纹 M- CG/E- CGA- 4、M- CG/E- CGA- 12和 M- CGG/E- GGC- 14 ,并探讨了应用 AFLSeventeen primer pairs of MseⅠ and EcoRⅠ were selected for AFLP (amplified fragment length polymorphism) fingerprint analysis in soybeans. The AFLP fingerprints of 92 accessions of annual wild soybean (Glycine soja) and cultivated soybean (Glycine max) collected from different ecological regions in China were established. The characteristic bands between G.soja and G.max were found as CG/E CGA 4, CG/CGA 12 and CGG/GGC 14. The feasibility of AFLP fingerprint in accession identification was discussed.

关 键 词：野生大豆 栽培大豆 扩增片段长度多态性 AFLP 指纹图谱 

分 类 号：S565.1[农业科学—作物学]