皮肤鳞状细胞癌转移灶中基质金属蛋白酶的表达  

Expression of matrix metalloproteinases in metastatic lesions of cutaneous squamous cell carcinoma

作　　者：陈翔[1] 金藏拓郎[2] 张桂英[1] 周瑛[1] 肖嵘[1] 李干群[1] 苏玉文[1] 颜兰香[1] 文海泉[1] 神崎保[2] 

机构地区：[1]中南大学湘雅二医院,长沙410011 [2]鹿儿岛大学医学部皮肤科

出　　处：《湖南医科大学学报》2001年第4期297-300,共4页Bulletin of Hunan Medical University

基　　金：国家自然科学基金资助项目 ( 2 0 0 40 0 2 8)

摘　　要：目的 :探讨皮肤鳞状细胞癌 (SCC)转移病灶中基质金属蛋白酶 (MMPs)的表达及其与basigin/CD147表达间的关系。方法 :运用免疫组化法检测 14例转移SCC中MMPs和basigin/CD147的表达 ,并通过免疫印迹法证实basi gin/CD147在转移SCC中的表达。结果 :正常皮肤和淋巴结中基本无MMPs表达 ,而 14例转移SCC的肿瘤细胞和基质细胞中均有MMPs的表达增高。 14例转移SCC中的 8例肿瘤细胞上basigin/CD147阳性染色 ,4例有强阳性染色。肿瘤细胞中basigin/CD147表达与基质细胞中MMP 1,MMP 2 ,MMP 3和MTI MMP的表达间均存在显著相关性 (P值分别为 0 0 12 ,0 0 2 4,0 0 47和 0 0 2 6 )。免疫印迹法证实转移SCC中basigin/CD147的表达增高。结论 :肿瘤细胞和基质细胞来源的MMPs均在皮肤鳞癌转移灶中发挥作用 ,肿瘤细胞中的basigin/CD147可能诱导基质细胞中MMPs的增高表达。Objective To investigate the expression of matrix metalloproteinases (MMPs) and its correlation with basigin/CD147 expression in metastatic lesions of cutaneous Squamous cell carcinoma (SCC). Methods Expressions of MMPs and basigin/CD147 were examined by DAB and ALP immunohistochemical staining in 14 metastatic SSCs, and immunoblot analysis was used to observe the basigin/CD147 expression in metastatic samples. Results Normal skin and lymph nodes were basically negative for MMPs, but expressions of MMP 1, MMP 2, MMP 3 and MTl MMP were increased in both tumor cells and stromal cells in metastatic SCC. Strongly positive or positive staining was observed in 4 or 8 of metastatic SCCs for basigin/CD147 in tumor cells respectively. Significant correlations were found between basigin/CD147 expression in tumor cells and MMP 1, MMP 2, MMP 3, MTl MMP expression in surrounding stromal cells (p values: 0.012, 0.024, 0.047 and 0.026, respectively). Immunoblot analysis revealed that basigin/CD147 expression increased in metastatic SCC. Conclusion These results suggest that MMPs produced by both tumor cells and stromal cells play a role in metastasis of cutaneous SCC, and increased MMPs in stromal cells might be induced by basigin/CD147 expressed in tumor cells.

关 键 词：基质金属蛋白酶 basigin/CD147 肿瘤转移 皮肤鳞状细胞癌 

分 类 号：R739.5[医药卫生—肿瘤]