益气解毒颗粒对大鼠鼻咽癌变中端粒酶和端粒酶RNA表达的影响  

Inhibitory effect of YIQIJIEDU powder to telomerase and telomerase RNA in nasopharyngeal tumorigenesis of rats

作　　者：唐发清[1] 田道法[2] 陈朝晖[1] 段朝军[3] 吴尚辉[3] 谌兵来[1] 荆照政[1] 

机构地区：[1]中南大学湘雅医院中心实验室,长沙410008 [2]湖南中医学院第一附属医院,长沙410007 [3]中南大学湘雅医学院肿瘤研究所,长沙410078

出　　处：《湖南医科大学学报》2001年第4期301-304,共4页Bulletin of Hunan Medical University

基　　金：国家自然科学基金资助项目 ( 39770 933) ;中南大学杰出青年基金

摘　　要：目的 :探讨中药复方益气解毒颗粒防治鼻咽癌的作用机制。方法 :用二亚硝基哌嗪 (DNP)诱导大鼠鼻咽癌动物模型 ,观察益气解毒颗粒对大鼠鼻咽癌变中病理形态改变以及端粒酶和端粒酶RNA表达的影响。定期分批处死大鼠 ,取鼻咽组织作病理学检测 ,用PCR -ELISA和NestedRT -PCR法检测大鼠鼻咽组织端粒酶的活性和端粒酶RNA的表达。结果 :灌服益气解毒颗粒大鼠鼻咽癌发癌率 0 (0 /5 ) ,明显低于盐水对照组 80 % (4/5 ) (包括原位癌和浸润癌 ) ;其端粒酶活性为 0 2 4± 0 11,也明显低于对照组 0 93± 0 2 3(P <0 .0 5 ) ;维甲酸组发病率为 0 (0 /5 ) ,端粒酶活性为 0 2 7± 0 13;各组端粒酶RNA均为阳性。维甲酸组大鼠于 2 15～ 30 8d间逐步死亡。结论 :益气解毒颗粒能阻断DNP诱导大鼠鼻咽癌变 。Objective Telomerase activation is a common event in malignant transformation, and is associated with tumorigenesis. This has been clarified in nasopharyngeal carcinoma. YIQIJIEDU powder (Chinese traditional medicine) is used to treated the patients with nasopharynegeal carcinoma (NPC) after radio-therapy and patients with precancerous lesion of NPC. We investigated whether YIQIJIEDU powder inhibits the telomersae activity in nasopharyngeal carcinogenesis. Methods Nasopharyngeal carcinomas in rats were induced by N, N' Dinitrosopiperazine (DNP), and some of them were treated with YIQIJIEDU powder. Telomerase and telomerase RNA in the rats' nasopharyngeal tissue were assayed by using PCR-ELISSA and Nested-PCR, and were pathologically diagnozed. Results YIQIJIEDU powder could inhibit telomerase activity and nasopharyngeal carcinogenesis in the nasopharyngeal tissue of the rats treated, but no telomerase RNA. Conclusion The findings suggest that the anticancer effect of YIQIJIEDU powder links with their inhibitory effects on telomerase.

关 键 词：鼻咽肿瘤 癌变 端粒酶 端粒酶RNA 益气解毒颗粒 大鼠 中医药治疗 

分 类 号：R739.63[医药卫生—肿瘤]