rasP^(21)、GST-π在胃癌及癌前病变组织表达的研究  

Immunohistochemical Study of rasP^(21) and GST-π Expressions in Gastirc Precancerous Lessions and Adenocarcinomas

作　　者：张运贵[1] 张钢锋[1] 王桂荣[1] 黄咏梅[1] 薛强[2] 宗传贞[2] 姚桂芹[1] 

机构地区：[1]泰山医学院第二附属医院消化科,济南250031 [2]泰山医学院第二附属医院病理科,济南250031

出　　处：《临床消化病杂志》2001年第6期264-266,共3页Chinese Journal of Clinical Gastroenterology

摘　　要：目的:观察ｒａｓＰ２１、ＧＳＴ－π在胃癌及癌前病变组织中的表达,探讨复合表达在胃粘膜癌变过程中的意义。方法:应用免疫组化(Ｓ－Ｐ)法测定４０例肠上皮化生(不全结肠型),７６例异型增生(轻度２０例,中度３４例,重度２２例)和４２例胃癌组织中ｒａｓＰ２１、ＧＳＴ－π的表达及复合表达情况,以１０例萎缩性胃炎胃粘膜做对照。结果:(１)ｒａｓＰ２１、ＧＳＴ－π在萎缩性胃炎中无阳性表达,在肠上皮化生、异型增生及胃癌中表达率及表达强度逐渐增高(Ｐ＜０．０５或０．０１);在轻、中、重度异型增生中表达率及表达强度逐渐增高(Ｐ＜０．０５或０．０１);在重度异型增生中表达率及表达强度与胃癌无显著性差异(Ｐ均＞０．０５)。(２)ｒａｓＰ２１、ＧＳＴ－π在肠上皮化生、异型增生及胃癌中复合表达率逐渐增高(Ｐ＜０．０１);在轻、中、重度异型增生中复合表达率逐渐增高(Ｐ＜０．０５);在重度异型增生与胃癌的复合表达率无显著差异(Ｐ＞０．０５)。结论:ｒａｓＰ２１、ＧＳＴ－π均与胃粘膜癌变过程有关,联合检测对胃癌的早期诊断有重要意义。Purpose: In order to diagnose gastric carcinomas earlier, we studied the expressions of rasP21 protein and placental form of glutathione stransferase (GST-π) in gastric precancerous lessions and adenocarcinomas, to investigate the coherent relation and significance of the two markers in the process of gastric mucosal carcinogenesis. Methods: Expression of rasP21 protein and GST-π were examined in 40 intestinal metaplasia (incomplete colonal type), 76 gastric mucosal atypical hyperplasia(slight 20, modrate 34, severe 22) and 42 gastric adenocarcinomas by immunohistochemical technique, with S-P method using monoclonal anti-rasP21 and anti-GST-π antibodies, compared with 10 atrophic gastritis membrane. Results: (1) rasP21 and GST-π positive staining rate and intensity were gradually increased among intestinal metaplasia, atypical hyperplasia and gastric adenocarcinomas, respectively ( P <C 0. 05 or 0. 01), but not expressed in atrophic gastritis membrane. Positive staining rate and intensity were gradully increased also among slight, moderate and severe atypical hyperplasia, respectively(P<0.05 or 0.01), but there were no significant differences of positive staining rate and intensity between severe atypical hyperplasia and gastric adenocarcinomas(P >0.05). (2)rasP21 and GST-π co-expression rate were gradually increased among intestinal metaplasia, atypical hyperplasia and gastric adenocarcinomas (P < 0. 01), and also among slight, modrate and severe atypical hyperplasia, but there were no significant differences of positive staining rate and intensity between severe atypical hyperplasia and gastric adenocarcinomas( P >0. 05). Conclusions: rasP21, GST-π participate in the process of gastric mucosal carcinogenesis, and detection of the co-expression have a significant value in the diagnosis of early gastric carcinomas.

关 键 词：RASP^21 GST-Π 不全结肠型肠上皮化生 异型增生 胃腺癌 

分 类 号：R735.2[医药卫生—肿瘤]