脾原发性淋巴瘤超微病理诊断及流式细胞分析的研究  

Ultrapathological Diagnosis and FCM Analyze of Primary Malignent Spleen Lymphoma

作　　者：脱朝伟[1] 刘秋珍[2] 王斌 吴泽全[3] 王明耀[2] 

机构地区：[1]解放军第202医院超微病理研究室,辽宁沈阳110003 [2]解放军第202医院超微病理研究室肝胆普外科,辽宁沈阳110003 [3]第三军医大学成都军医学院

出　　处：《中国医学影像技术》2001年第12期1185-1187,共3页Chinese Journal of Medical Imaging Technology

基　　金："九五"国家重点科技攻关计划基金资助项目(96A2 30 60 3)

摘　　要：目的　探讨诊断电镜 (DEM)对脾原发性淋巴瘤的病理分型诊断 ,流式细胞术 (FCM )检测瘤细胞DNA含量与其恶性程度及预后关系。方法　应用透射电镜和流式细胞术观察分析 41例脾原发性淋巴瘤超微结构和瘤细胞DNA含量。结果　 41例脾原发性淋巴瘤中霍奇金B细胞性淋巴瘤 5例占 12 .2 0 % ,非霍奇金B细胞性淋巴瘤 2 1例 ,占 5 1.2 2 % ;非霍奇金T免疫母细胞淋巴瘤 11例 ,占 2 6 .83% ;脾组织细胞性淋巴瘤 3例 ,占 7.31% ;伴毛细胞淋巴瘤1例 ,占 2 .34 %。FCM范围在 0 .772 .0 6 ,DI均值 1.12± 0 .37,其中二倍体肿瘤 13例 ,占 31.7% ;异倍体肿瘤 2 8例 ,占6 8.3%。低恶性组为 13例 ,DI为 1.12± 0 .37,中恶组 17例 ,DI为 1.45± 0 .2 5 ,高恶组 11例 ,DI为 1.71± 0 .19。低、中与高恶组相比 ,差异显著 (P <0 .0 1)。DI和超微病理组织学分型呈正相关关系。结论　DEM为脾原发性淋巴瘤超微病理诊断提供了形态学标准 ,FCM脾淋巴瘤细胞DNA倍性 。Objective To investigate the relationship among the ultrapathological differiation, DNA content and prophylaxis. Methods 41 cases of primary spleen lymphoma were studied by DEM and FCM. Results In 41 cases, B cell Hodgkin's lymphoma is 5(12.2%), B cell Non Hodgkin's lymphoma is 21(51.22%), Timmunoblastic Non Hodgkin's lymphoma is 11(26.83%),T cell immunoblast cell lyphoma is 3(7.31%), and spienic Lymphoma with circulating villous lymphocytes is 1 (2.43%).The rang of FCM is from 0.77 to 2.06, mean value of DI is 1.12±0.37, Doubleploid tumor is13(31.7%), hypertriploid is 28(68.3%). Low malignant DI are13 cases, (1.12±0.37); middle malignant are 17,(DI 1.45±0.37); high malignant are 11 (DI 1.71±0.19).Comparing with the levels of high malignant group, low and middle malignant groups are significant different ( P <0.01). DI is passively ralative to the pothohistological differiation. Conclusion DEM can offer morphological evidence for diagnosing primary spleen lymphoma. Using FCM to detect the DNA ploidy tumor cells, prolifering activation is helpful to identify the primary spleen lymphoma.

关 键 词：脾肿瘤 恶性淋巴瘤 超微结构 流式细胞术 诊断 

分 类 号：R733.204[医药卫生—肿瘤]