三氧化二砷对白血病细胞DNA损伤及凋亡相关基因表达的影响  

Effects of As_2O_3 on DNA damage and expression of apoptotic associated gene in leukemia

作　　者：欧英贤[1] 魏亚明[1] 

机构地区：[1]兰州军区兰州总医院血液病研究所,甘肃兰州730050

出　　处：《西北国防医学杂志》2002年第1期4-6,T001,共4页Medical Journal of National Defending Forces in Northwest China

基　　金：甘肃省自然科学基金资助项目 (ZS0 0 1-A2 3-0 82 -Y)

摘　　要：目的 :研究三氧化二砷 (As2 O3 )对HL - 6 0 ,K5 6 2和NB4细胞DNA的损伤及对凋亡相关基因的调控。方法 :用荧光显微技术 ,单细胞彗星电泳观察As2 O3 对HL - 6 0 ,K5 6 2及NB4细胞DNA的损伤 ,用流式细胞术测定As2 O3 对HL - 6 0 ,K5 6 2及NB4细胞凋亡相关基因Bcl- 2和p5 3的表达。结果 :As2 O3 诱导HL - 6 0 ,K5 6 2和NB4细胞的凋亡时造成了DNA损伤 ;显著下调三种细胞内Bcl- 2蛋白的表达 ,且Bcl- 2下调程度与凋亡有着密切关系 ;上调了p5 3蛋白的表达。结论 :As2 O3 诱导细胞凋亡时发生了DNA的损伤 ,诱导凋亡主要是通过下调Bcl- 2和上调p5 3来实现。Objective:To investigate the effects of As 2O 3 on DNA damage and expression of apoptotic associated gene in leukemia cell lines NB4, K562 and HL-60.Methods:Fluorescence microscope and single cell comet electrophoresis were used to detect apoptosis and DNA damage, expression of apoptotic associated gene products Bcl-2 and p53 were measured with flow cytometry as well. Results:DNA were damaged when As 2O 3 induced apoptosis among three leukemia cell lines, the expression of Bcl-2 protein were markedly down-regulated and p53 protein were up-regulated with the increase of As 2O 3 concentration during these process. Conclusion:Apoptosis induced by As 2O 3 mainly associated with down-regulation of Bcl-2 and up-regulation of p53 as well as damaged cell DNA.

关 键 词：白血病 三氧化二砷 DNA损伤 凋亡 

分 类 号：R733.7[医药卫生—肿瘤]