鸡传染性法氏囊病病毒基因组A片段cDNA序列分析  

Sequence Analysis of Chicken Infectious Bursal Disease Virus Ts Strain

作　　者：刘小军[1] 陈楠[1] 陈立栋[1] 陈永福[1] 

机构地区：[1]中国农业大学农业生物技术国家重点实验室,北京100094

出　　处：《畜牧兽医学报》2002年第1期85-88,共4页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金资助项目 ( 39770 5 6 0 )

摘　　要：用鸡胚成纤维细胞繁殖鸡传染性法氏囊病病毒 (IBDV)天水毒株。用提纯的病毒颗粒提取基因组RNA。根据已报道的英国 5 2 /70株的序列设计引物 ,用反转录 聚合酶链式反应 (RT PCR)进行cDNA扩增 ,获得 15 5 8bp和 15 90bp两个部分重叠的片段。结果表明 ,所克隆片段为 30 99的IBDV大开放读框。经与已报道的多个毒株相应序列比较后发现 ,核苷酸同源性介于 97 4%～ 99 8%之间 ,推导的氨基酸同源性介于 98 1%～ 99 5 %。The infectious bursal disease virus Ts strain was propagated in 10 day old specific pathogen free chicken embryo fibroblasts.Viral dsRNA was extracted and purified.Two pairs of primers were designed according to the segment A sequence of IBDV 52/70 strain reported previously.Two separate but overlapping cDNA fragments in length of 1558 bp and 1590 bp were synthesized and amplified by RT PCR.The PCR products were cloned into pGEM T vector and further subcloned into the vector pGEM 7zf.Sequencing showed that the larger ORF of IBDV Ts strain shared 97 4% 99 8% nucleotide sequence homology and 98 1% 99 5% deduced amino acid sequence homology with the published sequences of several other strains.

关 键 词：鸡 传染性法氏病毒 基因组A片段 序列分析 

分 类 号：S852.659.4[农业科学—基础兽医学] S858.315.3[农业科学—兽医学]