荧光定量聚合酶链反应检测乳腺癌外周血微小转移  

Detection of mammaglobin mRNA expression in peripheral blood by FQ-PCR in breast cancer patients with micrometastasis

作　　者：刘宁[1] 张伟[2] 郝希山[1] 李强[1] 王洪平[2] 金玉生[2] 刘毅[2] 曲平[2] 李茉[2] 邵永孚[3] 

机构地区：[1]天津医科大学肿瘤医院腹部外科,300060 [2]中国医学科学院肿瘤医院肿瘤研究所 [3]中国医学科学院肿瘤医院腹外科

出　　处：《中华普通外科杂志》2002年第1期38-40,共3页Chinese Journal of General Surgery

摘　　要：目的探讨应用荧光定量聚合酶链反应 (FQ PCR)扩增乳腺组织特异性基因hMAMmRNA作为检测乳腺癌血行微小转移方法的可能性。方法利用逆转录RT PCR检测hMAMmRNA在乳腺癌细胞系SKBR3的表达 ,将FQ PCR与普通PCR的敏感性进行比较 ,并利用FQ PCR对乳腺癌等不同病种患者 10 1例及健康志愿者 31例的外周血进行检测 ,分析hMAMmRNA表达与乳腺癌临床特点之间的关系。结果普通巢式PCR扩增hMAMmRNA在 10 6个白细胞中可检测出 1个SKBR3细胞 ,而巢式FQ PCR可在 10 7个白细胞中可检测出 1个SKBR3细胞 ,6 3例乳腺癌患者外周血中 19例hMAMmRNA阳性 (阳性率 30 % ) ,随肿瘤分期进展阳性率增高。其他病种患者外周血中均阴性 ,而 31例健康献血员中有 1例阳性。结论巢式FQObjectiveTo evaluate mammaglobin (hMAM) mRNA expression as a tumor marker for micrometastatic carcinoma cells in the peripheral blood of patients with breast cancer (BC). MethodsTotal RNA extracted from a breast carcinoma cell line SKBR3 was subjected to analysis of hMAM mRNA expression by RT-PCR. The sensitivity of FQ-PCR to detect a SKBR3 cell at a level of 10 7 was established. Peripheral blood of 63 BC patients was examined by this method. ResultsBlood samples were positive in 19 out of 63 (30%) patients with breast carcinomas. None of the patients with other cancer (25 cases) or benign breast disease (13 cases) was positive and only 1 out of 31 healthy volunteers was found with detectable hMAM mRNA expression in the peripheral blood. ConclusionFluorescence quantitative (FQ) PCR combined with nest PCR was a sensitive method in detecting micrometastases of breast cancer.

关 键 词：乳腺肿瘤 肿瘤转移 聚合酶链反应 RNA 放射免疫检测 FQ-PCR 

分 类 号：R737.9[医药卫生—肿瘤]