水溶性β-淀粉样蛋白25-35片段诱致大鼠嗜铬细胞瘤细胞凋亡  

Soluble Amyloid Beta Protein25-35 Induces Rat Pheochromacytoma Cells Apoptosis

作　　者：邵梅[1] 陈生弟[1] 刘振国[1] 

机构地区：[1]上海第二医科大学瑞金医院神经科,上海200025

出　　处：《中国神经免疫学和神经病学杂志》2002年第1期24-28,共5页Chinese Journal of Neuroimmunology and Neurology

基　　金：上海市科委自然科学基金面上资助项目 ( 99ZB14 0 19)

摘　　要：目的　探讨β-淀粉样蛋白 ( Aβ)在形成淀粉样沉积前对体外培养的 PC1 2细胞的毒性作用。方法　应用流式细胞仪检测 Aβ的剂量依赖性毒性 ;应用 DNA琼脂糖凝胶电泳、DNA末端标记和电镜判断 Aβ损伤细胞的途径。结果　流式细胞仪检测发现随着 Aβ2 5 -35浓度的增加 ,PC1 2细胞的死亡率增加 ;加入 1 0 μmol/L Aβ2 5 -35 2 4 h后 ,经 TUNEL发现细胞核着色 ,并可见细胞核碎片 ;DNA琼脂糖凝胶电泳可见间隔 1 80～ 2 0 0 bp左右的梯度条带 ;电镜显示细胞核浓缩 ,胞浆内有空泡形成 ,胞浆内细胞器完好。结论　 Aβ在形成纤维状沉积前即对神经细胞有毒性作用 ,可促进神经细胞的凋亡。Objective To study the neurotoxicity of amyloid beta protein on cultured PC12 cells before it aggregated into fibrillary protein.Methods The dose manner neurotoxicity of Aβ on PC12 cells was detected by flow cytometry, and the type of PC12 cell death was investigated by TUNEL staining, eletron microscope and DNA electrophoresis.Results With the increasing concentration of Aβ25 35 ,the ratio of cell death increased. 24 hours after 10 μmol/L Aβ25 35 treatment, TUNEL staining was positive, DNA electrophoresis showed DNA ladder , and electron microscope showed the nuclear condensation and bubbling in the cytoplasma. Conclusions Aβ demonstrated obvious neurotoxicity on PC12 cells before it aggregated, and induced PC12 cells apoptosis.

关 键 词：可溶性淀粉样蛋白25-35片段 大鼠嗜铬细胞瘤细胞 凋亡 阿尔茨海默病 

分 类 号：R749.16[医药卫生—神经病学与精神病学]