rhGM-CSF联合rhIL-4对髓性白血病细胞B7分子表达的上调作用  

In Vitro Upregulation of B7 Molecules Expression by rhGM?CSF and rhIL?4 in Myeloid Leukemia Cells

作　　者：陈勤奋[1] 谢彦晖[1] 戴振声[1] 陈彤[1] 徐晓武[1] 谢弘[2] 谢毅[1] 

机构地区：[1]复旦大学附属华山医院血液科,上海200040 [2]中国科学院上海细胞生物学研究所,上海200031

出　　处：《复旦学报（医学版）》2001年第5期390-392,共3页Fudan University Journal of Medical Sciences

基　　金：原上海医科大学校内基金资助项目

摘　　要：目的　探讨GM CSF联合IL 4对髓性白血病细胞表面B7分子表达的上调作用。方法　从初诊急性或慢性髓性白血病患者的外周血中分离出单个核细胞 (PBMNC) ,用rhGM CSF联合rhIL 4共同孵育 7～ 10d ;通过流式细胞仪检测细胞因子诱导前后细胞表面B7分子的表达。结果　未经细胞因子诱导的白血病细胞表面B7分子低表达或缺如 ,经过GM CSF联合IL 47～ 10d的诱导 ,可显著上调B7分子的表达。结论　髓性白血病细胞表面存在B7分子表达缺陷 ;用GM CSF联合ILPurpose: To investigate the upregulation efficacy of rhGM-CSF and rhIL-4 in B7 costimulatory molecules expression on myeloid leukemia cells. Methods: MNCs were isolated from peripheral blood of acute and chronic myeloid leukemia patients, co-cultured with rhGM-CSF and rhIL-4 for 7 - 10 days. Before and after cytokines induced, B7-1 (CD80) and B7-2 (CD86) expression was assayed by flow cytometry. Results: Before induced, average B7-1 and B7-2 expression was (1.20 ± 0.79)% and (3.69 ± 2.23)%, respectively. After 7 days cytokines co-cultured, average epression was (8.98 ± 2.98)% and (22.24 ± 5.07)%, respectively. And after 10 days co-cultured, the expression was (6.58 ± 3.11) % and (14.90 ± 5.73)%, respectively. Conclusions: B7 costimulatory molecules expression on the myeloid leukemia cell surface was defective. These costimulatory molecules on the myeloid leukemia cells can be upregulated by a oombination of rhGM-CSF and rhIL-4.

关 键 词：B7分子 共刺激分子 髓性白血病 细胞因子 RHGM-CSF rhIL-4 

分 类 号：R733.7[医药卫生—肿瘤]