三氯乙烯接触工人外周血淋巴细胞染色体畸变分析  

An analysis of chromosome aberrations in peripheral lymphocytes in the workers exposed to tricholoroethylene

作　　者：邓丽霞[1] 陈雯[1] 徐雷[1] 杨杏芬[1] 胡斌[1] 郑履康[1] 

机构地区：[1]中山医科大学公共卫生学院劳动卫生职业病学教研室,广东广州510089

出　　处：《中国工业医学杂志》2001年第5期275-277,共3页Chinese Journal of Industrial Medicine

摘　　要：目的　探讨三氯乙烯 (TCE)对接触工人有否致淋巴细胞染色体畸变作用。方法　选接触TCE工人 32名 ,另选对照工人 30名。用人外周血体外培养法检测淋巴细胞染色体畸变 (CA)率 ,并用气相色谱法测定车间空气中TCE浓度和分光光度吡啶法测定尿中三氯乙酸 (TCA)含量。结果　车间空气中TCE浓度分别为 41 2mg/m3和83 1mg/m3,均超过国家最高容许浓度 ,接触组尿中TCA含量为对照组的 81 5倍 ,并与个体接触TCE浓度成正相关(r =0 761,P <0 0 0 1) ;接触组人外周血淋巴细胞CA率 ( 1 15± 0 84) %与对照组CA率 ( 1 0 6± 0 78) %比较差异无显著性 (P >0 0 5 )。结论　未见接触TCE工人外周血淋巴细胞染色体畸变率增加。接触人群尿中TCA含量随接触TCE浓度的增加而升高 ,有较好的相关 ,提示暴露者尿中TCA含量能反映TCE的接触水平。Objectives To explore whether tricholoroethylene(TCE)could cause chromosome aberrations in the exposed workers.Methods Thirty-two workers exposed to TCE and 30 control workers were selected.In vitro culture method was applied to detect the frequency of lymphocyte aberrations in peripheral lymphocytes.Air concentration of TCE in the workplace was monitored with gas chromatography and urine trichloroacetic acid(TCA)was determined with pyridine spectrophotometry.Results The air concentrations of TCE in two workplaces was 41.2 mg/m 3 and 83.1 mg/m 3,higher than the national maximum allowable concentration.Urine level of TCA in the exposed group was 81.5 times high as in the control one,which correlated with the TCE concentrations they exposed,with r=0.761,P<0.001.Chromosome aberration rate was(1.15±0.84)% in the exposed group,not significantly different from that in the control one(1.06±0.78)%(P<0.05).Conclusion No increase in chromosome aberrations in peripheral lymphocyte in the TCE-exposed workers was found.Urine TCA level in the exposed group increased with the concentrations they exposed,suggesting it could be used an indicator reflecting exposure to TCE.

关 键 词：三氯乙烯 现场调查 染色体畸变 TCE 淋巴细胞 

分 类 号：R557.4[医药卫生—血液循环系统疾病] O623.221[医药卫生—内科学]